Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Hepatobiliary & Pancreatic Surgery and Liver Transplantation, Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran.
Can J Gastroenterol Hepatol. 2021 Apr 23;2021:5593655. doi: 10.1155/2021/5593655. eCollection 2021.
Several animal studies have shown the roles of cytokines in regulating liver regeneration following liver resection (LR), which is a type of surgery designed to remove cancerous tumors from the liver. This study investigated how the expressions and serum levels of some pro- and anti-inflammatory cytokines in patients with hepatocellular carcinoma (HCC) were changed during LR.
Liver tissues from 15 patients with HCC were collected and the levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-), IL-1, IL-1 , IL-10, and transforming growth factor-beta1 (TGF-1) were assessed using real-time PCR assay at different times before and after LR. The serum values of TNF- and IL-6 were also measured by ELISA.
After 60 and 90 minutes of LR, IL-6 gene expression was significantly increased ( < 0.001 - 0.05). The same trend was also observed in TNF- expression after 90 minutes of LR ( < 0.01). No significant changes were observed in the expressions of IL-1, IL-1, IL-10, and TGF-1 before and after LR. In addition, LR had significant effects on TNF- and IL-6 serum levels ( < 0.05 - 0.0001).
Our data provided further evidence to reveal that IL-6 and TNF- cytokines are critical to improve liver regeneration.
多项动物研究表明细胞因子在肝切除（LR）后肝再生中的作用，LR 是一种旨在从肝脏中切除癌性肿瘤的手术。本研究旨在探讨肝细胞癌（HCC）患者 LR 过程中某些促炎和抗炎细胞因子的表达和血清水平如何变化。
收集 15 例 HCC 患者的肝组织，在 LR 前后不同时间点采用实时 PCR 法检测白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、IL-1β、IL-1ra、IL-10 和转化生长因子-β1（TGF-β1）的水平。还通过 ELISA 法测量 TNF-α和 IL-6 的血清值。
LR 后 60 和 90 分钟，IL-6 基因表达显著增加（<0.001-0.05）。LR 后 90 分钟，TNF-α的表达也呈现相同的趋势（<0.01）。LR 前后，IL-1β、IL-1ra、IL-10 和 TGF-β1 的表达无明显变化。此外，LR 对 TNF-α和 IL-6 的血清水平有显著影响（<0.05-0.0001）。
我们的数据进一步提供了证据，表明 IL-6 和 TNF-α细胞因子对改善肝再生至关重要。
