Hsiao Hung-Tsung, Chien Hsiao-Jung, Lin Ya-Chi, Liu Yen-Chin
Department of Anesthesiology, E-Da Hospital, E-Da University, Kaohsiung City, Taiwan.
Department of Anesthesiology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan City, Taiwan.
Kaohsiung J Med Sci. 2017 Apr;33(4):165-170. doi: 10.1016/j.kjms.2017.01.009. Epub 2017 Feb 28.
Transcutaneous electrical nerve stimulators (TENSs) have been proved to be effective in muscle pain management for several decades. However, there is no consensus for the optimal TENS program. Previous research demonstrated that a 100 Hz TENS (L-TENS) provided better analgesia than a conventional TENS (< 5 Hz). However, no research compared a higher-frequency (> 100 Hz) TENS with a 100 Hz TENS. We used a 5000 Hz (5 kHz) frequency TENS (M-TENS) and an L-TENS to compare analgesic effect on a mice skin/muscle incision retraction model. Three groups of mice were used (sham, L-TENS, and M-TENS) and applied with different TENS programs on Day 4 after the mice skin/muscle incision retraction model; TENS therapy was continued as 20 min/d for 3 days. Mice analgesic effects were measured via Von Frey microfilaments with the up-down method. After therapy, mice spinal cord dorsal horn and dorsal root ganglion (DRG) were harvested for cytokine evaluation (tumor necrosis factor-α and interleukin-1β) with the Western blotting method. Our data demonstrated that the M-TENS produced better analgesia than the L-TENS. Cytokine in the spinal cord or DRG all expressed lower than that of the sham group. However, there is no difference in both cytokine levels between TENSs of different frequencies in the spinal cord and DRG. We concluded that the M-TENS produced faster and better mechanical analgesia than the L-TENS in the mice skin/muscle incision retraction model. Those behavior differences were not in accordance with cytokine changes in the spinal cord or DRG.
几十年来，经皮电神经刺激仪（TENS）已被证明在肌肉疼痛管理方面有效。然而，对于最佳TENS方案尚无共识。先前的研究表明，100Hz的TENS（L-TENS）比传统的TENS（<5Hz）提供更好的镇痛效果。然而，没有研究将更高频率（>100Hz）的TENS与100Hz的TENS进行比较。我们使用5000Hz（5kHz）频率的TENS（M-TENS）和L-TENS来比较对小鼠皮肤/肌肉切口回缩模型的镇痛效果。使用三组小鼠（假手术组、L-TENS组和M-TENS组），并在小鼠皮肤/肌肉切口回缩模型建立后的第4天应用不同的TENS方案；TENS治疗持续20分钟/天，共3天。通过采用序贯法的von Frey微丝测定小鼠的镇痛效果。治疗后，采集小鼠脊髓背角和背根神经节（DRG），采用蛋白质印迹法评估细胞因子（肿瘤坏死因子-α和白细胞介素-1β）。我们的数据表明，M-TENS比L-TENS产生更好的镇痛效果。脊髓或DRG中的细胞因子表达均低于假手术组。然而，脊髓和DRG中不同频率TENS的两种细胞因子水平没有差异。我们得出结论，在小鼠皮肤/肌肉切口回缩模型中，M-TENS比L-TENS产生更快、更好的机械性镇痛效果。这些行为差异与脊髓或DRG中的细胞因子变化不一致。
