Regueira M S, Tintino Saulo Relison, da Silva Ana Raquel Pereira, Costa Maria do Socorro, Boligon Aline Augusti, Matias Edinardo F F, de Queiroz Balbino Valdir, Menezes Irwin R A, Melo Coutinho Henrique Douglas
Departamento de Genética, Centro de Ciências Biológicas, UFPE, Brazil.
Departamento de Química Biológica, Centro de Ciências Biológicas e Saúde, Universidade Regional do Cariri, Brazil.
Food Chem Toxicol. 2017 Sep;107(Pt B):572-580. doi: 10.1016/j.fct.2017.03.052. Epub 2017 Mar 27.
The aim of this study was to investigate the effect of the dry and rainy season on the antibacterial activity and chemical composition of the Brazilian red propolis. The samples were collected in rainy (RP-PER) and dry (RP-PED) seasons and analyzed by HPLC-DAD. The extracts were tested alone and in association with antibiotics against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The HPLC analysis identified luteolin and quercetin as the main compounds. Seasonal variation was observed according to concentrations of the compounds. The MIC values against E. coli ranged from 128 μg/mL to 512 μg/mL (EC 06 and EC ATCC). The red propolis showed MIC values of 512 μg/mL against both strains of P. aeruginosa used in our study (PA03 and PA24) and against strains of Gram-positive bacteria S. aureus the MICs ranged from 64 μg/mL to ≥1024 μg/mL (SA10). A synergistic effect was observed when we combined the RP-PED with gentamicin against all the strains tested. When we combined the RP-PED with Imipenem, we only observed synergistic effect against P. aeruginosa. According to our synergistic activity results, the utilization of red propolis collected in the drier periods can be used as an adjuvant against multiresistant bacterial infections.
本研究的目的是调查旱季和雨季对巴西红蜂胶抗菌活性和化学成分的影响。在雨季（RP-PER）和旱季（RP-PED）采集样本，并通过高效液相色谱-二极管阵列检测器（HPLC-DAD）进行分析。提取物单独以及与抗生素联合用于测试对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的抗菌效果。HPLC分析确定木犀草素和槲皮素为主要化合物。观察到化合物浓度存在季节性变化。对大肠杆菌的最低抑菌浓度（MIC）值范围为128μg/mL至512μg/mL（EC 06和EC ATCC）。红蜂胶对本研究中使用的两种铜绿假单胞菌菌株（PA03和PA24）的MIC值均为512μg/mL，对革兰氏阳性菌金黄色葡萄球菌菌株的MIC值范围为64μg/mL至≥1024μg/mL（SA10）。当将RP-PED与庆大霉素联合使用时，对所有测试菌株均观察到协同作用。当将RP-PED与亚胺培南联合使用时，仅观察到对铜绿假单胞菌的协同作用。根据我们的协同活性结果，利用在较干燥时期采集的红蜂胶可作为抗多重耐药细菌感染的佐剂。
