Bueno-Silva Bruno, Marsola Alexandre, Ikegaki Masaharu, Alencar Severino M, Rosalen Pedro L
a Dental Research Division , Guarulhos University , Guarulhos , Brazil.
b Piracicaba Dental School , State University of Campinas - UNICAMP , Piracicaba , Brazil.
Nat Prod Res. 2017 Jun;31(11):1318-1324. doi: 10.1080/14786419.2016.1239088. Epub 2016 Oct 4.
The aim of this study was to evaluate the effect of seasons on the chemical composition and antibacterial activity of Brazilian red propolis (BRP) and its plant source. BRP was collected from Maceio, Alagoas state, north-east of Brazil, during one year. Chemical composition was determined by physicochemical analyses and HPLC while antimicrobial activity was assessed against Streptococcus mutans, Streptococcus sobrinus, Staphylococcus aureus and Actinomyces naeslundii by determining the minimal inhibitory and bactericidal concentrations (MIC and MBC, respectively). The comparative chemical profiles varied quantitatively according to the collection period. Formononetin was the most abundant compound in both propolis and resin, while isoliquiritigenin, (3S)-neovestitol, (3S)-vestitol are suggested to be responsible for antimicrobial activity of Brazilian red propolis. MIC varied from 15.6 to 125 μg/mL, whereas MBC varied from 31.2 to 500 μg/mL. Therefore, season in which propolis and its botanical source are collected indeed influences their chemical compositions, resulting in variations in their antibacterial activity.
本研究旨在评估季节对巴西红蜂胶（BRP）及其植物来源的化学成分和抗菌活性的影响。在一年的时间里，从巴西东北部阿拉戈斯州马塞约采集了BRP。通过物理化学分析和高效液相色谱法测定化学成分，同时通过测定最小抑菌浓度和杀菌浓度（分别为MIC和MBC）来评估对变形链球菌、远缘链球菌、金黄色葡萄球菌和内氏放线菌的抗菌活性。比较化学图谱根据采集时期在数量上有所不同。在蜂胶和树脂中，刺芒柄花素是最丰富的化合物，而异甘草素、（3S）-新vestitol、（3S）-vestitol被认为是巴西红蜂胶抗菌活性的原因。MIC在15.6至125μg/mL之间变化，而MBC在31.2至500μg/mL之间变化。因此，采集蜂胶及其植物来源的季节确实会影响它们的化学成分，从而导致其抗菌活性的变化。
