Chen Guang Feng, Wu Ru Gang, Li Dong Mei, Yu Hai Xia, Deng Zhiying, Tian Ji Chun
State Key Laboratory of Crop Biology, Group of Quality Wheat Breeding of Shandong Agricultural University, Tai'an 271018, People's Republic of China.
J Genet. 2017 Mar;96(1):177-186. doi: 10.1007/s12041-016-0731-1.
Seeding emergence and tiller number are the most important traits for wheat (Triticum aestivum L.) yield, but the inheritance of seeding emergence and tillering is poorly understood. We conducted a genomewide association study focussing on seeding emergence and tiller number at different growth stages with a panel of 205 elite winter wheat accessions. The population was genotyped with a high-density Illumina iSelect 90K SNPs assay. A total of 31 loci were found to be associated with seeding emergence rate (SER) and tiller number in different growth stages. Loci distributed among 12 chromosomes accounted for 5.35 to 11.33% of the observed phenotypic variation. With this information, 10 stable SNPs were identified for eventual development of cleaved amplified polymorphic sequence markers for SER and tiller number in different growth stages. Additionally, a set of elite alleles were identified, such as Ra_c14761_1348-T, which may increase SER by 13.35%, and Excalibur_c11045_236-A and BobWhite_c8436_391-T, which may increase the rate of available tillering by 14.78 and 8.47%, respectively. These results should provide valuable information for marker-assisted selection and parental selection in wheat breeding programmes.
种子出苗率和分蘖数是小麦（Triticum aestivum L.）产量的最重要性状，但对种子出苗和分蘖的遗传了解甚少。我们利用一组205份优良冬小麦种质进行了全基因组关联研究，重点关注不同生长阶段的种子出苗率和分蘖数。该群体通过高密度Illumina iSelect 90K SNP分析进行基因分型。共发现31个位点与不同生长阶段的种子出苗率（SER）和分蘖数相关。分布在12条染色体上的位点占观察到的表型变异的5.35%至11.33%。利用这些信息，鉴定出10个稳定的SNP，最终用于开发不同生长阶段SER和分蘖数的酶切扩增多态性序列标记。此外，还鉴定出一组优良等位基因，如Ra_c14761_1348-T，可使SER提高13.35%；Excalibur_c11045_236-A和BobWhite_c8436_391-T，可分别使有效分蘖率提高14.78%和8.47%。这些结果应为小麦育种计划中的标记辅助选择和亲本选择提供有价值的信息。
