酵母/单纯疱疹病毒胸苷激酶基因融合产生具有胸苷激酶活性的融合蛋白。
Yeast/herpes simplex virus thymidine kinase gene fusions yield fusion proteins with thymidine kinase activity.
作者信息
McNeil J B, Little J G
机构信息
Department of Biology, York University, Downsview, Ontario, Canada.
出版信息
Curr Genet. 1985;9(7):567-72. doi: 10.1007/BF00381169.
PMID:2836094
Abstract
Expression in Saccharomyces cerevisiae of the Herpes Simplex Virus type-1 (HSV-1) thymidine kinase gene was accomplished by the construction of a gene fusion between the TK and a yeast gene. The fusion of yeast DNA sequences (which include a promoter and DNA that codes for the amino terminus end of the yeast gene product) with the TK gene resulted in a protein fusion with thymidine kinase activity.
摘要
单纯疱疹病毒1型（HSV-1）胸苷激酶基因在酿酒酵母中的表达是通过构建TK与酵母基因之间的基因融合来实现的。酵母DNA序列（包括一个启动子和编码酵母基因产物氨基末端的DNA）与TK基因的融合产生了具有胸苷激酶活性的蛋白质融合体。
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