Kiss G B, Pearlman R E, Cornish K V, Friesen J D, Chan V L
J Bacteriol. 1982 Feb;149(2):542-7. doi: 10.1128/jb.149.2.542-547.1982.
The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kinase coding sequence was detected.
单纯疱疹病毒胸苷激酶基因已被克隆到一种嵌合酵母质粒克隆载体中,并转化到合适的酵母菌株中。携带单纯疱疹病毒胸苷激酶基因的质粒可以作为自主复制质粒进行增殖,但未检测到胸苷激酶编码序列特异性的RNA。