Gonzalez-Perez Francisco, Cobianchi Stefano, Heimann Claudia, Phillips James B, Udina Esther, Navarro Xavier
Institute of Neurosciences and Department of Cell Biology, Physiology and Immunology, UniversitatAutònoma de Barcelona, and CIBERNED, Spain.
Medovent GmbH, Mainz, Germany.
Neurosurgery. 2017 Mar 1;80(3):465-474. doi: 10.1093/neuros/nyw068.
Autograft is still the gold standard technique for the repair of long peripheral nerve injuries. The addition of biologically active scaffolds into the lumen of conduits to mimic the endoneurium of peripheral nerves may increase the final outcome of artificial nerve devices. Furthermore, the control of the orientation of the collagen fibers may provide some longitudinal guidance architecture providing a higher level of mesoscale tissue structure.
To evaluate the regenerative capabilities of chitosan conduits enriched with extracellular matrix-based scaffolds to bridge a critical gap of 15 mm in the rat sciatic nerve.
The right sciatic nerve of female Wistar Hannover rats was repaired with chitosan tubes functionalized with extracellular matrix-based scaffolds fully hydrated or stabilized and rolled to bridge a 15 mm nerve gap. Recovery was evaluated by means of electrophysiology and algesimetry tests and histological analysis 4 months after injury.
Stabilized constructs enhanced the success of regeneration compared with fully hydrated scaffolds. Moreover, fibronectin-enriched scaffolds increased muscle reinnervation and number of myelinated fibers compared with laminin-enriched constructs.
A mixed combination of collagen and fibronectin may be a promising internal filler for neural conduits for the repair of peripheral nerve injuries, and their stabilization may increase the quality of regeneration over long gaps.
自体移植仍然是修复长段周围神经损伤的金标准技术。在导管管腔内添加生物活性支架以模拟周围神经的神经内膜，可能会提高人工神经装置的最终效果。此外，控制胶原纤维的取向可提供一些纵向引导结构，从而提供更高水平的中尺度组织结构。
评估富含基于细胞外基质的支架的壳聚糖导管修复大鼠坐骨神经15 mm关键缺损的再生能力。
用完全水合或稳定化并卷绕以桥接15 mm神经缺损的基于细胞外基质的支架功能化的壳聚糖管修复雌性Wistar Hannover大鼠的右侧坐骨神经。损伤4个月后，通过电生理学、痛觉测定试验和组织学分析评估恢复情况。
与完全水合的支架相比，稳定化构建体提高了再生成功率。此外，与富含层粘连蛋白的构建体相比，富含纤连蛋白的支架增加了肌肉再支配和有髓纤维数量。
胶原蛋白和纤连蛋白的混合组合可能是用于修复周围神经损伤的神经导管的一种有前景的内部填充物，并且它们的稳定化可能会提高长间隙再生的质量。
