修饰甲基化酶M. SinI的分离与特性分析
Isolation and characterization of the modification methylase M . SinI.
作者信息
Karreman C, de Waard A
机构信息
Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, The Netherlands.
出版信息
J Bacteriol. 1988 Jun;170(6):2533-6. doi: 10.1128/jb.170.6.2533-2536.1988.
A sequence-specific modification methylase (M . SinI) was isolated and purified from Escherichia coli harboring a derivative of recombinant plasmid pSI4 (see accompanying manuscript: C. Karreman and A. de Waard, J. Bacteriol. 170:2527-2532, 1988), which contains a Salmonella infantis DNA insert. The enzyme uniquely methylates the internal deoxycytidylate residue in the nucleotide sequence GG(A/T)MeCC, thereby protecting DNA completely against cleavage by restriction endonuclease R . SinI or R . AvaII [GG(A/T)CC], and in part against cleavage by R . Sau96I (GGNCC).
从携带重组质粒pSI4衍生物的大肠杆菌中分离并纯化出一种序列特异性修饰甲基化酶（M. SinI）（见随附文稿：C. Karreman和A. de Waard，《细菌学杂志》170:2527 - 2532，1988），该质粒含有婴儿沙门氏菌DNA插入片段。该酶能特异性地甲基化核苷酸序列GG(A/T)MeCC中的内部脱氧胞苷酸残基，从而使DNA完全免受限制性内切酶R. SinI或R. AvaII [GG(A/T)CC]的切割，并部分免受R. Sau96I（GGNCC）的切割。
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