来自金黄色葡萄球菌的第二种位点特异性限制性内切酶。
A second site-specific restriction endonuclease from Staphylococcus aureus.
作者信息
Sussenbach J S, Steenbergh P H, Rost J A, van Leeuwen W J, van Embden J D
出版信息
Nucleic Acids Res. 1978 Apr;5(4):1153-63. doi: 10.1093/nar/5.4.1153.
Abstract
A site-specific restriction endonuclease has been isolated from Staphylococcus aureus PS 96. This enzyme, Sau96 I, recognizes the DNA sequence 5'--G-G-N-C-C--3' and cleaves as indicated by the arrows. The enzyme 3'--C-C-N-G-G--5' cleaves adenovirus type 5 and lambda DNA many times, SV40 DNA 10 times and 0X174 RF DNA 2 times. Evidence is presented that the enzyme is involved in biological restriction-modification.
摘要
一种位点特异性限制内切酶已从金黄色葡萄球菌PS 96中分离出来。这种酶，即Sau96 I，识别DNA序列5'--G-G-N-C-C--3'，并按箭头所示进行切割。该酶3'--C-C-N-G-G--5'可多次切割5型腺病毒和λ噬菌体DNA，切割SV40 DNA 10次，切割0X174 RF DNA 2次。有证据表明该酶参与生物限制修饰作用。
Zotero 插件