一种脱氧胞苷酸甲基转移酶的分离，该酶能够独特地保护DNA免受核酸内切酶R.Aqu I（Ava I的同裂酶）的切割。
Isolation of a deoxycytidylate methyl transferase capable of protecting DNA uniquely against cleavage by endonuclease R.Aqu I (isoschizomer of Ava I).
作者信息
Karreman C, Tandeau de Marsac N, de Waard A
出版信息
Nucleic Acids Res. 1986 Jul 11;14(13):5199-205. doi: 10.1093/nar/14.13.5199.
Abstract
A sequence-specific modification methylase (M.AquI) was isolated and purified from Agmenellum quadruplicatum (Synechococcus PCC 7002). This enzyme uniquely methylates the deoxycytidylate residue in the sequence *CYCGRG indicated by the asterisk. It was shown to protect DNA against cleavage by restriction endonucleases AvaI, SmaI and XhoI, which recognize the sequences CYCGRG, CCCGGG, and CTCGAG, respectively.
摘要
从四倍体聚球藻（聚球藻属PCC 7002）中分离并纯化出一种序列特异性修饰甲基化酶（M.AquI）。这种酶能特异性地甲基化星号所示*CYCGRG序列中的脱氧胞苷酸残基。研究表明，它能保护DNA不被分别识别CYCGRG、CCCGGG和CTCGAG序列的限制性内切酶AvaI、SmaI和XhoI切割。
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