Heidarizadeh Mohammad, Doustkhah Esmail, Rostamnia Sadegh, Rezaei Parisa Fathi, Harzevili Farshad Darvishi, Zeynizadeh Behzad
Organic and Nano Group (ONG), Department of Chemistry, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran; Department of Microbiology, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran.
Organic and Nano Group (ONG), Department of Chemistry, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran; Department of Chemistry, Faculty of Science, Urmia University, Urmia 57159-165, Iran.
Int J Biol Macromol. 2017 Aug;101:696-702. doi: 10.1016/j.ijbiomac.2017.03.152. Epub 2017 Mar 29.
Immobilization of lipase was successfully achieved on the surface of magnetically separable FeO/graphene oxide (GO) via a post-modification. This post modification was achieved in alternation to glutaraldehyde post-modification. The activity of immobilized lipase had not a significant loss in the activity while on the other hand, it is simply extractable (by keeping its major activity) from reaction crude by a magnet. Each step of immobilization was carefully monitored by characterization and all were successfully proved. SEM, TEM, XRD, EDX, and FTIR were used to characterize the support and immobilization process.
通过后修饰成功地将脂肪酶固定在磁性可分离的FeO/氧化石墨烯（GO）表面。这种后修饰是在戊二醛后修饰的基础上交替进行的。固定化脂肪酶的活性没有显著损失，另一方面，它可以通过磁铁从反应粗产物中简单地提取出来（同时保持其主要活性）。固定化的每一步都通过表征进行了仔细监测，并且所有结果都得到了成功验证。使用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X射线衍射（XRD）、能量散射X射线光谱（EDX）和傅里叶变换红外光谱（FTIR）对载体和固定化过程进行了表征。
