Department of Chemistry, Faculty of Science, Payame Noor University, PO Box 19395-3697, Tehran, Iran.
Organic and Nano Group (ONG), Department of Chemistry, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran; Department of Microbiology, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran.
Int J Biol Macromol. 2017 Oct;103:1194-1200. doi: 10.1016/j.ijbiomac.2017.05.159. Epub 2017 Jun 1.
In the present paper, porcine pancreas lipase (PPL) was immobilized to a new version of magnetite via a novel stepwise dithiocarbamate/chitosan-based method in alternation to glutaraldehyde. Magnetic chitosan nanocomposite was post-modified to produce dithiocarbamate moieties on the surface through amine functions. Then, immobilization of lipase was successfully achieved on the surface of magnetically separable FeO@CS/NHCSH via a post-modification. Each step of immobilization was carefully monitored by characterization and all were successfully proved. Comparison of immobilized enzyme with free enzyme showed that the method of immobilization is efficient.
在本文中，猪胰腺脂肪酶（PPL）通过一种新的逐步二硫代氨基甲酸盐/壳聚糖方法固定在新型磁铁上，取代了戊二醛。磁性壳聚糖纳米复合材料经过后修饰，通过胺基在表面产生二硫代氨基甲酸盐部分。然后，通过后修饰成功地将脂肪酶固定在可分离的 FeO@CS/NHCSH 表面上。通过特征化仔细监测每个固定化步骤，并且所有步骤都成功得到证实。固定化酶与游离酶的比较表明，该固定化方法是有效的。
