Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.
Biosens Bioelectron. 2017 Aug 15;94:575-583. doi: 10.1016/j.bios.2017.03.050. Epub 2017 Mar 23.
In this work, we report a novel iridium(III)-based luminescent switch on-off-on probe, for the in vitro and in vivo detection of sulfide ion. The mechanism of this platform is based on the effective charge transfer quenching of the iridium(III) complex 1 by Fe, followed by the restoration of luminescence upon the addition of NaS. The probe, hereinafter referred to as 1-Fe, exhibited a linear range of detection for NaS from 0.01 to 1.5mM, with a detection limit of 2.9μM at signal-to-noise ratio (S/N) of 3. We also demonstrate the utility of 1-Fe for cell-based imaging as well as for the detection of enzymatic sulfide generation in living zebrafish.
在这项工作中，我们报道了一种新型的基于铱(III)的发光开关型探针，用于体外和体内检测硫化物离子。该平台的机制基于铱(III)配合物 1 与 Fe 的有效电荷转移猝灭，随后加入 NaS 恢复发光。该探针，以下简称 1-Fe，对 NaS 的检测范围从 0.01 到 1.5mM 呈线性，在信噪比(S/N)为 3 时，检测限为 2.9μM。我们还证明了 1-Fe 用于基于细胞的成像以及在活斑马鱼中检测酶促硫化物生成的实用性。
