Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China.
School of Foreign Languages, Kunming University, Kunming 650200, China.
Bioresour Technol. 2017 Jul;235:104-112. doi: 10.1016/j.biortech.2017.03.114. Epub 2017 Mar 22.
Microalgae lipids are potential candidates for the production of renewable biodiesel. The combination of plant hormones and two-step cultivation regulates lipid production in microalgae. A strategy for promoting lipid accumulation in Monoraphidium sp. QLY-1 by combining exogenous melatonin (MT) and photoinduction was developed. The effects of melatonin on the lipid content, reactive oxygen species (ROS), and activities of three key fatty acid biosynthetic enzyme in Monoraphidium sp. QLY-1 were investigated. The lipid content increased by 1.32-fold under 1μM melatonin treatment. The maximum lipid content achieved was 49.6%. However, the protein and carbohydrate contents decreased rapidly from 57.21% to 47.96% and from 53.4% to 37.71%, respectively. Biochemical and physiological analyses suggested that the ROS and lipid biosynthesis-related enzyme activities correlated with increased lipid accumulation under photo-melatonin induction conditions.
微藻油脂是生产可再生生物柴油的潜在候选物。植物激素与两步培养相结合，调节微藻中的油脂生产。通过组合外源性褪黑素（MT）和光诱导，开发了一种促进单胞藻 QLY-1 中脂类积累的策略。研究了褪黑素对单胞藻 QLY-1 中脂类含量、活性氧（ROS）和三种关键脂肪酸生物合成酶活性的影响。在 1μM 褪黑素处理下，脂质含量增加了 1.32 倍。最大脂质含量达到 49.6%。然而，蛋白质和碳水化合物含量迅速从 57.21%降至 47.96%和从 53.4%降至 37.71%。生化和生理分析表明，在光-褪黑素诱导条件下，ROS 和与脂质生物合成相关的酶活性与脂质积累增加相关。
