通过诱导独脚金内酯提高单枝藻 QLY-1 中的脂类积累。
Enhancement of lipid accumulation in Monoraphidium sp. QLY-1 by induction of strigolactone.
机构信息
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
出版信息
Bioresour Technol. 2019 Sep;288:121607. doi: 10.1016/j.biortech.2019.121607. Epub 2019 Jun 4.
In this study, the effects of strigolactone (SL) on the biomass, lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The lipid content and lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca and lipid biosynthesis gene transcription. Subsequently, the relationship between Ca and nitric oxide (NO) in the regulation of cell growth and lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca regulated cell growth and lipid biosynthesis by modulating the levels of NO and lipid biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca and NO signalling in the manipulation of lipid biosynthesis in microalgae under SL treatment.
在这项研究中，研究了独脚金内酯（SL）对 Monoraphidium sp.QLY-1 的生物量、脂质含量、生化特性和基因转录的影响。与对照组相比，在 1μM SL 诱导下，QLY-1 的脂质含量和脂质生产力分别增加了 61%和 55%。SL 还上调了内源性 NO 和 Ca 的水平以及脂质生物合成基因的转录。随后，分析了 SL 诱导条件下 Ca 和一氧化氮（NO）在调节 QLY-1 细胞生长和脂质积累中的关系。内源性 Ca 的增加通过调节 NO 水平和脂质生物合成相关基因表达来调节细胞生长和脂质生物合成。总的来说，这项研究为 SL 诱导下微藻生物燃料的生产提供了一种有价值的方法，并表明在 SL 处理下操纵微藻中的脂质生物合成时，Ca 和 NO 信号之间存在关键的串扰。
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