褪黑素通过多层次机制增强缺氮条件下 Monoraphidium sp. QLY-1 的脂类生产。
Melatonin enhances lipid production in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions via a multi-level mechanism.
机构信息
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
School of Foreign Languages, Kunming University, Kunming 650200, China.
出版信息
Bioresour Technol. 2018 Jul;259:46-53. doi: 10.1016/j.biortech.2018.03.014. Epub 2018 Mar 13.
In this study, melatonin (MT) promoted lipid accumulation in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions. The lipid accumulation increased 1.22- and 1.36-fold compared with a nitrogen-starved medium and a normal BG-11 medium, respectively. The maximum lipid content was 51.38%. The reactive oxygen species (ROS) level in the presence of melatonin was lower than that in the control group, likely because of the high antioxidant activities. The application of melatonin upregulated the gibberellin acid (GA) production and rbcL and accD expression levels but downregulated the abscisic acid (ABA) content and pepc expression levels. These findings demonstrated that exogenous melatonin could further improve the lipid production in Monoraphidium sp. QLY-1 by regulating antioxidant systems, signalling molecules, and lipid biosynthesis-related gene expression under nitrogen deficiency conditions.
在这项研究中，褪黑素（MT）促进了缺氮条件下的单胞藻 QLY-1 中的脂质积累。与氮饥饿培养基和正常 BG-11 培养基相比，脂质积累分别增加了 1.22 倍和 1.36 倍。最大脂质含量为 51.38%。在褪黑素存在的情况下，活性氧（ROS）水平低于对照组，这可能是因为抗氧化活性较高。褪黑素的应用上调了赤霉素（GA）的产生以及 rbcL 和 accD 的表达水平，但下调了脱落酸（ABA）的含量和 pepc 的表达水平。这些发现表明，外源性褪黑素可以通过调节抗氧化系统、信号分子和与脂质生物合成相关的基因表达，进一步提高缺氮条件下单胞藻 QLY-1 的脂质产量。
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