Kohl S, West M S, Loo L S
Program in Infectious Diseases and Clinical Microbiology, University of Texas Medical School Biometry Program, Houston.
J Pediatr. 1988 Jun;112(6):976-81. doi: 10.1016/s0022-3476(88)80229-4.
Natural killer cytotoxicity (NKC) is an important early defense mechanism in viral infections. We determined the ability of interleukin-2 (IL-2), an NKC stimulator, to enhance defective neonatal NKC to virus-infected cells. Human recombinant IL-2-stimulated adult and cord blood NKC to herpes simplex virus-infected cells in a time-dependent and dose-dependent fashion. The highest level of neonatal IL-2-stimulated cytotoxicity approached the level of unstimulated cytotoxicity when adult cells are used. Single-cell experiments suggested that the cord blood defect was due not to decreased adherence but to lysis or recycling defects. IL-2 stimulated adhesion in the presence of antibody but had no stimulatory effect on antibody-dependent cellular cytotoxicity. The relative defects in IL-2 stimulation of neonatal NKC suggest that its lone use as a therapeutic or protective agent against herpes simplex virus infections is unlikely to be successful, and may require concomitant adult cells if NKC is a critical mechanism.
自然杀伤细胞细胞毒性（NKC）是病毒感染中一种重要的早期防御机制。我们测定了NKC刺激物白细胞介素-2（IL-2）增强缺陷新生儿NKC对病毒感染细胞作用的能力。重组人IL-2以时间和剂量依赖的方式刺激成人及脐血NKC对单纯疱疹病毒感染细胞的作用。当使用成人细胞时，新生儿IL-2刺激的细胞毒性最高水平接近未刺激的细胞毒性水平。单细胞实验表明，脐血的缺陷并非由于黏附减少，而是由于裂解或再循环缺陷。IL-2在抗体存在的情况下刺激黏附，但对抗体依赖性细胞毒性没有刺激作用。新生儿NKC的IL-2刺激相对缺陷表明，单独将其用作抗单纯疱疹病毒感染的治疗或保护剂不太可能成功，如果NKC是关键机制，可能需要同时使用成人细胞。
