Kohl S, Frazier J J, Greenberg S B, Pickering L K, Loo L S
J Pediatr. 1981 Mar;98(3):379-84. doi: 10.1016/s0022-3476(81)80699-3.
Low natural killer cytotoxicity has been associated with serious herpes simplex virus infections. Neonates' mononuclear cells had significantly lower NKC to HSV infected (P less than 0.001) and uninfected (P less than 0.005) target cells than did adults' MC. Under appropriate conditions, both neonates' MC to NKC was increased by exogenous human leukocyte interferon. Although neonates' and adults' MC had a similar sensitivity to interferon, there was a significant (P = 0.05) lack of consistency in neonates' MC response. Twenty-three percent (4/17) of neonates' MC samples did not respond with increased NKC in the presence of interferon, unlike adults' MC, all of which demonstrated increased NKC. Neonates' MC did not suppress adults' MC-NKC. These data demonstrate the efficacy of interferon as an NKC stimulator in the neonate, but indicate a heterogeneous response of neonatal cells. We are encouraged with the prospect of clinical trials of interferon to treat severe viral infections in neonates. We urge the inclusion of immune surveillance in these trials.
自然杀伤细胞（NKC）细胞毒性低下与严重的单纯疱疹病毒感染有关。新生儿的单核细胞对单纯疱疹病毒感染的靶细胞（P<0.001）和未感染的靶细胞（P<0.005）的NKC显著低于成人的单核细胞（MC）。在适当条件下，外源性人白细胞干扰素可增加新生儿MC向NKC的转化。虽然新生儿和成人的MC对干扰素的敏感性相似，但新生儿MC反应存在显著（P = 0.05）的不一致性。与成人的MC不同，成人的MC在干扰素存在下均表现出NKC增加，而23%（4/17）的新生儿MC样本在干扰素存在下并未出现NKC增加。新生儿的MC并未抑制成人的MC-NKC。这些数据证明了干扰素作为新生儿NKC刺激剂的有效性，但表明新生儿细胞存在异质性反应。我们对干扰素治疗新生儿严重病毒感染的临床试验前景感到鼓舞。我们敦促在这些试验中纳入免疫监测。
