School of Chemical Engineering, Taishan Medical University, Taian, Shandong 271016, PR China.
School of Chemical Engineering, Taishan Medical University, Taian, Shandong 271016, PR China.
Anal Chim Acta. 2017 May 1;965:103-110. doi: 10.1016/j.aca.2017.02.006. Epub 2017 Feb 20.
A novel fluorophore pyrido[1,2-a]benzimidazole was synthesized and used as a donor to construct FRET system. The probe shows a large Stokes shifts (190 nm), high selectivity, fast response (within 2 min), and high sensitivity toward Cu (detection limit 42 nM). A significant colour change from colourless to pink could be observed, enabling naked-eye detection of Cu. Furthermore, it could be used for imaging Cu in living cells which demonstrated its value of practical applications in biological systems.
合成了一种新型荧光团吡啶并[1,2-a]苯并咪唑，并将其用作供体构建 FRET 体系。该探针具有较大的斯托克斯位移（190nm）、高选择性、快速响应（在 2min 内）和对 Cu 的高灵敏度（检测限为 42nM）。可以观察到从无色到粉红色的明显颜色变化，实现了 Cu 的裸眼检测。此外，它还可用于活细胞中 Cu 的成像，这表明其在生物体系中的实际应用价值。
