Yu Jianjun, Liu Yan, Guo Can, Zhang Shanshan, Gong Zhaojian, Tang Yanyan, Yang Liting, He Yi, Lian Yu, Li Xiayu, Deng Hao, Liao Qianjin, Li Xiaoling, Li Yong, Li Guiyuan, Zeng Zhaoyang, Xiong Wei, Yang Xinming
Department of Head and Neck Surgery, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China;; Department of Otorhinolaryngology Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China;; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Cancer Research Institute, Central South University, Changsha, Hunan, China.
Department of Head and Neck Surgery, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China;; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Cancer Research Institute, Central South University, Changsha, Hunan, China.
J Cancer. 2017 Feb 11;8(4):523-530. doi: 10.7150/jca.17510. eCollection 2017.
Altered expression of long non-coding RNAs (lncRNAs) associated with human carcinogenesis and might be used as diagnosis and prognosis biomarkers. However, the expression of lncRNAs in tongue squamous cell carcinoma (TSCC) and their relevance on the diagnosis, progression and prognosis of TSCC have not been thoroughly elucidated. To discover novel TSCC-related lncRNAs, we analyzed the lncRNA expression patterns in two sets of previously published TSCC gene expression profile data (GSE30784 and GSE9844), and found that long intergenic non-coding RNA 152 (LINC00152) was significantly upregulated in TSCC samples. We then detected LINC00152 expression in two other cohorts of TSCC samples. Quantitative Real time PCR (qRT-PCR) results indicated that LINC00152 was highly expressed in 15 primary TSCC biopsies when compared with 14 adjacent non-tumor tongue squamous cell epithelium samples. The expression of LINC00152 was also measured in 182 paraffin-embedded human TSCC tissues by hybridization, increased expression of LINC00152 was significantly correlated with TSCC progression, such as T stage ( = 0.009), N stage ( = 0.036), TNM stage ( = 0.017), and associated with relapse ( < 0.001), and invasion ( < 0.001). Kaplan-Meier analysis demonstrated that increased LINC00152 expression contributed to both poor overall survival ( = 0.006) and disease-free survival ( = 0.007) of TSCC patients. These findings suggest that LINC00152 might serve as a potential biomarker for early detection and prognosis prediction of TSCC.
长链非编码RNA（lncRNAs）的表达改变与人类致癌作用相关，可能用作诊断和预后生物标志物。然而，lncRNAs在舌鳞状细胞癌（TSCC）中的表达及其与TSCC诊断、进展和预后的相关性尚未完全阐明。为了发现新的与TSCC相关的lncRNAs，我们分析了两组先前发表的TSCC基因表达谱数据（GSE30784和GSE9844）中的lncRNA表达模式，发现长链基因间非编码RNA 152（LINC00152）在TSCC样本中显著上调。然后我们在另外两组TSCC样本中检测了LINC00152的表达。定量实时PCR（qRT-PCR）结果表明，与14个相邻的非肿瘤舌鳞状细胞上皮样本相比，LINC00152在15个原发性TSCC活检组织中高表达。还通过杂交检测了182例石蜡包埋的人TSCC组织中LINC00152的表达，LINC00152表达增加与TSCC进展显著相关，如T分期（P = 0.009）、N分期（P = 0.036）、TNM分期（P = 0.017），并与复发（P < 0.001）和侵袭（P < 0.001）相关。Kaplan-Meier分析表明，LINC00152表达增加导致TSCC患者总体生存率差（P = 0.006）和无病生存率差（P = 0.007）。这些发现表明，LINC00152可能作为TSCC早期检测和预后预测的潜在生物标志物。
