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腺病毒主要晚期启动子的复制诱导刺激与一种因子与第一个内含子中序列的结合相关。

Replication-induced stimulation of the major late promoter of adenovirus is correlated to the binding of a factor to sequences in the first intron.

作者信息

Jansen-Durr P, Boeuf H, Kédinger C

机构信息

Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génetique de l'INSERM, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.

出版信息

Nucleic Acids Res. 1988 May 11;16(9):3771-86. doi: 10.1093/nar/16.9.3771.

DOI:10.1093/nar/16.9.3771
PMID:2836800
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336555/
Abstract

The sequence requirements for transcriptional stimulation of the adenovirus major late promoter (MLP) by the products of the early transcription unit Ela and by the replication of viral DNA were analyzed by in vitro transcription. Sequences upstream of +33 are involved in the moderate Ela-responsiveness of the MLP, while sequences between +33 and +131 are required for its major replication-induced transcriptional activation. Dnase I footprinting experiments delineate a sequence component, extending from +76 to +120, which binds protein(s) only in extracts of cells where viral DNA replication occurred. Taken together, these results suggest that the replication-dependent stimulation of the MLP is mediated by the increased binding of this protein(s).

摘要

通过体外转录分析了腺病毒主要晚期启动子（MLP）受早期转录单元E1a产物和病毒DNA复制的转录刺激的序列要求。+33上游的序列参与MLP的中度E1a反应性，而+33和+131之间的序列是其主要复制诱导的转录激活所必需的。DNA酶I足迹实验确定了一个从+76延伸到+120的序列成分，该成分仅在发生病毒DNA复制的细胞提取物中与蛋白质结合。综上所述，这些结果表明MLP的复制依赖性刺激是由这种蛋白质结合增加介导的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/336555/af282c7a58e0/nar00152-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/336555/e74f1b94c542/nar00152-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/336555/3a08518eca29/nar00152-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/336555/ce873e07a2d0/nar00152-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/336555/af282c7a58e0/nar00152-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/336555/e74f1b94c542/nar00152-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/336555/3a08518eca29/nar00152-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/336555/ce873e07a2d0/nar00152-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ff/336555/af282c7a58e0/nar00152-0187-a.jpg

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2
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本文引用的文献

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Transcripts from the adenovirus-2 major late promoter yield a single early family of 3' coterminal mRNAs and five late families.腺病毒2型主要晚期启动子的转录本产生一个3' 共末端mRNA的早期家族和五个晚期家族。
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DNA replication and the early to late transition in adenovirus infection.腺病毒感染中的DNA复制及早期到晚期的转变
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Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.
腺病毒L4-22K蛋白通过序列特异性单链RNA结合来调节转录和RNA剪接。
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The adenovirus L4 33-kilodalton protein binds to intragenic sequences of the major late promoter required for late phase-specific stimulation of transcription.腺病毒L4 33千道尔顿蛋白与主要晚期启动子的基因内序列结合，该启动子是晚期阶段特异性转录刺激所必需的。
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Viral DNA synthesis-dependent titration of a cellular repressor activates transcription of the human adenovirus type 2 IVa2 gene.细胞阻遏物的病毒DNA合成依赖性滴定激活人2型腺病毒IVa2基因的转录。
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Activation of the early-late switch in adenovirus type 5 major late transcription unit expression by L4 gene products.腺病毒5型主要晚期转录单位表达中L4基因产物对早期-晚期开关的激活作用。
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Encapsidation of viral DNA requires the adenovirus L1 52/55-kilodalton protein.病毒DNA的衣壳化需要腺病毒L1 52/55千道尔顿蛋白。
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Nucleic Acids Res. 1984 Oct 11;12(19):7423-33. doi: 10.1093/nar/12.19.7423.
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tk Enzyme expression in differentiating muscle cells is regulated through an internal segment of the cellular tk gene.在分化的肌肉细胞中，胸苷激酶（tk）酶的表达是通过细胞胸苷激酶基因的一个内部片段来调控的。
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