Thomas G P, Mathews M B
Cell. 1980 Nov;22(2 Pt 2):523-33. doi: 10.1016/0092-8674(80)90362-1.
Expression of the late genes of adenovirus is only detectable after virus DNA synthesis has occurred. Using a superinfection protocol, we show that replication of the template per se is required for expression of late regions L2--L5 (mapping to the right of position 39) and that the accumulation of early gene products does not suffice. This regulation is probably exerted at the level of transcription rather than by control of processing or selective stabilization of late mRNA or its precursors. The promoter-proximal late gene block L1, however, appears to be subject to processing control. At early times a single member of this gene family (tripartite leader plus coordinates 29--39, encoding the 52,55K polypeptide pair) is expressed, whereas at late times an additional, differently spliced mRNA species is generated from this region (tripartite leader plus coordinates 34--39, encoding polypeptide IIIa).
腺病毒晚期基因的表达只有在病毒DNA合成发生后才能检测到。使用一种超感染方案，我们发现晚期区域L2-L5（定位在39位右侧）的表达需要模板本身的复制，早期基因产物的积累是不够的。这种调控可能是在转录水平上发挥作用，而不是通过对晚期mRNA或其前体的加工或选择性稳定的控制。然而，启动子近端晚期基因块L1似乎受加工控制。在早期，这个基因家族的一个成员（三联体前导序列加上坐标29-39，编码52、55K多肽对）被表达，而在晚期，这个区域会产生另一种不同剪接的mRNA物种（三联体前导序列加上坐标34-39，编码多肽IIIa）。
