Crossland L D, Raskas H J
J Virol. 1983 Jun;46(3):737-48. doi: 10.1128/JVI.46.3.737-748.1983.
The relationship between adenovirus type 2 DNA replication and expression of intermediate stage viral genes was investigated. The 1.03-kilobase mRNA from early region 1b (E1b) and the mRNAs coding for proteins IX and IVa2 were first detected between 6 and 8 h postinfection. Inhibition of viral DNA replication with hydroxyurea prevented expression of the IX and IVa2 mRNAs, but not of the E1b mRNA. Pulse-labeling experiments demonstrated that the block of IX and IVa2 expression in hydroxyurea-treated cells was at the level of transcription. By a series of superinfection experiments, it was determined that the viral and cellular factors present during the late stage of adenovirus infection are insufficient to activate IX gene expression. The viral DNA template must first replicate before IX transcription can begin.
研究了2型腺病毒DNA复制与中期病毒基因表达之间的关系。感染后6至8小时首次检测到来自早期区域1b（E1b）的1.03千碱基mRNA以及编码蛋白质IX和IVa2的mRNA。用羟基脲抑制病毒DNA复制可阻止IX和IVa2 mRNA的表达，但不影响E1b mRNA的表达。脉冲标记实验表明，在羟基脲处理的细胞中，IX和IVa2表达的阻断发生在转录水平。通过一系列的超感染实验确定，腺病毒感染后期存在的病毒和细胞因子不足以激活IX基因的表达。病毒DNA模板必须先复制，IX转录才能开始。
