鸡胸苷激酶基因的核苷酸序列及其预测的多肽与痘苗病毒胸苷激酶多肽的关系。
The nucleotide sequence of the chicken thymidine kinase gene and the relationship of its predicted polypeptide to that of the vaccinia virus thymidine kinase.
作者信息
Kwoh T J, Engler J A
出版信息
Nucleic Acids Res. 1984 May 11;12(9):3959-71. doi: 10.1093/nar/12.9.3959.
Abstract
The entire DNA nucleotide sequence of a 3.0 kilobase pair Hind III fragment containing the chicken cytoplasmic thymidine kinase gene was determined. Oligonucleotide linker insertion mutations distributed throughout this gene and having known effects upon gene activity ( Kwoh , T.J., Zipser , D., and Wigler , M. 1983. J. Mol. Appl. Genet. 2, 191-200), were used to access regions of the Hind III fragment for sequencing reactions. The complete nucleotide sequence, together with the positions of the linker insertion mutations within the sequence, allows us to propose a structure for the chicken thymidine kinase gene. The protein coding sequence of the gene is divided into seven small segments (each less than 160 base pairs) by six small introns (each less than 230 base pairs). The proposed 244 amino acid polypeptide encoded by this gene bears strong homology to the vaccinia virus thymidine kinase. No homology with the thymidine kinases of the herpes simplex viruses was found.
摘要
测定了包含鸡细胞质胸苷激酶基因的一个3.0千碱基对Hind III片段的完整DNA核苷酸序列。利用分布于该基因中且对基因活性有已知影响的寡核苷酸接头插入突变（Kwoh，T.J.，Zipser，D.，以及Wigler，M. 1983.《分子与应用遗传学杂志》2，191 - 200）来确定用于测序反应的Hind III片段区域。完整的核苷酸序列，连同序列中接头插入突变的位置，使我们能够提出鸡胸苷激酶基因的结构。该基因的蛋白质编码序列被六个小内含子（每个小于230碱基对）分成七个小片段（每个小于160碱基对）。此基因编码的推测的244个氨基酸的多肽与痘苗病毒胸苷激酶有很强的同源性。未发现与单纯疱疹病毒胸苷激酶有同源性。
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