Azadmanesh Jahaun, Trickel Scott R, Weiss Kevin L, Coates Leighton, Borgstahl Gloria E O
Eppley Institute for Research in Cancer and Allied Diseases, 987696 Nebraska Medical Center, Omaha, NE 68198-7696, USA.
Biology and Soft Matter Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, TN 37831, USA.
Acta Crystallogr F Struct Biol Commun. 2017 Apr 1;73(Pt 4):235-240. doi: 10.1107/S2053230X17003508. Epub 2017 Mar 29.
Superoxide dismutases (SODs) are enzymes that protect against oxidative stress by dismutation of superoxide into oxygen and hydrogen peroxide through cyclic reduction and oxidation of the active-site metal. The complete enzymatic mechanisms of SODs are unknown since data on the positions of hydrogen are limited. Here, methods are presented for large crystal growth and neutron data collection of human manganese SOD (MnSOD) using perdeuteration and the MaNDi beamline at Oak Ridge National Laboratory. The crystal from which the human MnSOD data set was obtained is the crystal with the largest unit-cell edge (240 Å) from which data have been collected via neutron diffraction to sufficient resolution (2.30 Å) where hydrogen positions can be observed.
超氧化物歧化酶（SODs）是一类酶，通过活性位点金属的循环还原和氧化，将超氧化物歧化为氧气和过氧化氢，从而抵御氧化应激。由于关于氢位置的数据有限，SODs完整的酶促机制尚不清楚。本文介绍了使用全氘代法以及橡树岭国家实验室的MaNDi光束线进行人锰超氧化物歧化酶（MnSOD）大晶体生长和中子数据收集的方法。获得人MnSOD数据集的晶体是具有最大晶胞边长（240 Å）的晶体，通过中子衍射已收集到足够分辨率（2.30 Å）的数据，在此分辨率下可以观察到氢的位置。
