Jaques G, Rotsch M, Wegmann C, Worsch U, Maasberg M, Havemann K
Philipps University Medical Center, Division of Hematology/Oncology, Marburg, West Germany.
Exp Cell Res. 1988 Jun;176(2):336-43. doi: 10.1016/0014-4827(88)90335-7.
Small cell lung cancer (SCLC) cell lines were examined for the presence of insulin-like growth factor I-related protein (IGF-I) in cell pellets and culture media. IGF-I immunoreactivity was detected in 11/14 pellets, ranging from 12 to 76 mIU/mg soluble protein. The IGF-I levels in the cell pellets showed a correlation to the corresponding culture media. IGF-I binding sites were found in all tested cell lines. The maximum binding (Bmax) ranged from 131 to 1230 fmol/mg protein and the dissociation constant (KD) from 0.89 to 5.21 nM. The incorporation of [3H]thymidine in the presence of recombinant human IGF-I resulted in a clearly increased DNA synthesis in two of seven cell lines. Thus, IGF-I may be an important growth factor in SCLC.
对小细胞肺癌（SCLC）细胞系的细胞沉淀和培养基中胰岛素样生长因子I相关蛋白（IGF-I）的存在情况进行了检测。在14个细胞沉淀中有11个检测到IGF-I免疫反应性，可溶性蛋白含量范围为12至76 mIU/mg。细胞沉淀中的IGF-I水平与相应的培养基呈现相关性。在所有测试的细胞系中均发现了IGF-I结合位点。最大结合量（Bmax）范围为131至1230 fmol/mg蛋白，解离常数（KD）范围为0.89至5.21 nM。在重组人IGF-I存在的情况下，[3H]胸苷的掺入导致七个细胞系中的两个细胞系的DNA合成明显增加。因此，IGF-I可能是小细胞肺癌中的一种重要生长因子。
