Manivel J C, Jessurun J, Wick M R, Dehner L P
Am J Surg Pathol. 1987 Jan;11(1):21-9. doi: 10.1097/00000478-198701000-00003.
We analyzed the sensitivity of a polyclonal antibody to placental alkaline phosphatase (PLAP) in labelling testicular germ-cell neoplasms, by utilizing the peroxidase-antiperoxidase technique. The immunoreactivity of 89 germ-cell tumors for PLAP was as follows: 98% of cases with seminomatous elements were PLAP-positive; 97% of embryonal carcinomas and 85% of endodermal sinus tumors also showed reactivity. Cytotrophoblastic cells were focally immunoreactive in one of two cases with choriocarcinomatous elements. Staining for PLAP was strongest and most diffuse in seminomas. Intratubular germ-cell neoplasia (ITGCN) or carcinoma in situ was present in 53 (84%) of 63 specimens that had adjacent seminiferous tubules available for evaluation; PLAP was demonstrated in 98% of these. In addition, the germ-cell elements in 11 gonadoblastomas were immunoreactive for PLAP. One of 17 cases of undescended testes had ITGCN that was also strongly immunoreactive, but the remaining 16 cases were negative. Five dysgenetic gonads without ITGCN were studied, and one was immunoreactive for PLAP. Three testicular biopsy specimens from infertile men without ITGCN were PLAP-negative. Our findings indicate that PLAP is a highly sensitive marker for seminomas, for the majority of embryonal carcinomas and endodermal sinus tumors of the testis, and for ITGCN.
我们利用过氧化物酶-抗过氧化物酶技术分析了一种抗胎盘碱性磷酸酶（PLAP）多克隆抗体标记睾丸生殖细胞肿瘤的敏感性。89例生殖细胞肿瘤对PLAP的免疫反应性如下：含精原细胞瘤成分的病例中98%为PLAP阳性；97%的胚胎癌和85%的内胚窦瘤也显示出反应性。在两例含绒毛膜癌成分的病例中，有一例的细胞滋养层细胞呈局灶性免疫反应性。精原细胞瘤中PLAP染色最强且最弥漫。63份有相邻生精小管可供评估的标本中，53份（84%）存在小管内生殖细胞肿瘤（ITGCN）或原位癌；其中98%显示有PLAP。此外，11例性腺母细胞瘤中的生殖细胞成分对PLAP呈免疫反应性。17例隐睾病例中有1例存在ITGCN且也呈强免疫反应性，但其余16例为阴性。研究了5例无ITGCN的发育异常性腺，其中1例对PLAP呈免疫反应性。3例无ITGCN的不育男性的睾丸活检标本PLAP为阴性。我们的研究结果表明，PLAP是精原细胞瘤、大多数睾丸胚胎癌和内胚窦瘤以及ITGCN的高度敏感标志物。
