Yoruk O, Tatar A, Keles O N, Cakir A
Department of Otorhinolaryngology, Head and Neck Surgery, School of Medicine, Ataturk University, Erzurum, Turkey.
Department of Histology and Embryology, School of Medicine, Ataturk University, Erzurum, Turkey.
Acta Otorhinolaryngol Ital. 2017 Feb;37(1):32-37. doi: 10.14639/0392-100X-1143.
The aim of this study was to investigate the effect of Nigella sativa and cephalexin in the therapy of experimental bacterial rhinosinusitis. Bacterial rhinosinusitis was induced with Staphylococcus aureus. Rabbits were divided into five groups; control (n = 6), N. sativa 50 mg/ kg/d (n = 6), N. sativa 100 mg/kg/d (n = 6), N. sativa 200 mg/kg/d (n = 6), and cephalexin 20 mg/kg/d (n = 6) groups. N. sativa was given orally for 7 days. The same volume of normal saline (0.9% NaCl) was given as a vehicle to the control group for the same period. After treatment period, sinus mucosa samples were evaluated using stereological and histopathological methods. Half of the maxillary sinus mucosa samples were frozen at -800C for further analysis of NO levels. Pathology revealed a severe acute inflammatory process in rabbits treated with saline. Only mild inflammation was determined in cephalexin group, N. sativa 100 mg/kg/d and N. sativa 200 mg/kg/d groups. The level of NO increased in the saline group was significantly reduced in all treatment groups. N. sativa may prevent histopathological changes of rhinosinusitis via decreased NO levels in a dose dependent manner and can be used in the treatment of rhinosinusitis diseases.
本研究的目的是调查黑种草和头孢氨苄在实验性细菌性鼻窦炎治疗中的作用。用金黄色葡萄球菌诱发细菌性鼻窦炎。将兔子分为五组：对照组（n = 6）、黑种草50毫克/千克/天组（n = 6）、黑种草100毫克/千克/天组（n = 6）、黑种草200毫克/千克/天组（n = 6）和头孢氨苄20毫克/千克/天组（n = 6）。黑种草口服给药7天。同期给对照组给予相同体积的生理盐水（0.9%氯化钠）作为赋形剂。治疗期结束后，使用体视学和组织病理学方法评估鼻窦黏膜样本。将一半的上颌窦黏膜样本在-80℃冷冻以进一步分析一氧化氮水平。病理学显示，用生理盐水治疗的兔子出现严重的急性炎症过程。在头孢氨苄组、黑种草100毫克/千克/天组和黑种草200毫克/千克/天组仅确定有轻度炎症。生理盐水组中升高的一氧化氮水平在所有治疗组中均显著降低。黑种草可能通过以剂量依赖方式降低一氧化氮水平来预防鼻窦炎的组织病理学变化，并可用于治疗鼻窦炎疾病。
