Ahmad Bashir, Hafeez Nabia, Ara Gulshan, Azam Sadiq, Bashir Shumaila, Khan Ibrar
Centre of Biotechnology and Microbiology, University of Peshawar, Khyber Pakhtunkhwa, Pakistan.
Department of Pharmacy, University of Peshawar, Khyber Pakhtunkhwa, Pakistan.
Pak J Pharm Sci. 2016 Nov;29(6):1977-1983.
Staphylococcus aureus is a nosocomial pathogen that resides in the soft tissues causing many diseases. The current study was conducted to determine the prevalence of Methicillin Resistant S. aureus (MRSA) in ear discharge and pus of patients and antibacterial activity of crude methanolic extract (Cr. MeOH Ext.) and various fractions of M. Africana and V. agnus castus against clinical isolates of MRSA. A total of 40 samples were collected from ear, nose and throat (ENT) outpatient department and wards of Khyber Teaching Hospital (KTH), Peshawar. Out of 40 samples, 36 (90%) samples showed growth on Mannitol Salt Agar (MSA) media out of which 9(25%) were MRSA and the remaining 27(75%) were methicillin susceptible S. aureus (MSSA). A good antibacterial activity was observed for the Cr. MeOH Ext. (76.1%) and ethyl acetate (EtOAc) fraction of V. agnus castus against S11 (71.4%). The n-hexane fraction also showed good antibacterial effect (70%) against S. The chloroform (CHCl3), butanol (BuOH) and aqueous fractions of M. africana showed good antibacterial activity against S (71.4%), S32 (70%) and S (75%), respectively. The above results revealed that the selected plants can be further utilized for isolation of the active ingredients as the crude extracts were found good for inhibition of MRSA.
金黄色葡萄球菌是一种医院病原体，存在于软组织中，可引发多种疾病。本研究旨在确定患者耳分泌物和脓液中耐甲氧西林金黄色葡萄球菌（MRSA）的流行情况，以及非洲楝和贞洁树粗甲醇提取物（Cr. MeOH Ext.）及其不同馏分对MRSA临床分离株的抗菌活性。从白沙瓦开伯尔教学医院（KTH）的耳鼻喉科（ENT）门诊和病房共采集了40份样本。在40份样本中，36份（90%）在甘露醇盐琼脂（MSA）培养基上生长，其中9份（25%）为MRSA，其余27份（75%）为甲氧西林敏感金黄色葡萄球菌（MSSA）。观察到贞洁树的Cr. MeOH Ext.（76.1%）和乙酸乙酯（EtOAc）馏分对S11具有良好的抗菌活性（71.4%）。正己烷馏分对S也显示出良好的抗菌效果（70%）。非洲楝的氯仿（CHCl3）、丁醇（BuOH）和水馏分分别对S（71.4%）、S32（70%）和S（75%）显示出良好的抗菌活性。上述结果表明，由于发现粗提物对MRSA有良好的抑制作用，所选植物可进一步用于分离活性成分。
