Niazi Massumeh, Saki Mojgan, Sepahvand Maryam, Jahanbakhsh Sareh, Khatami Mehrdad, Beyranvand Mania
Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Ann Med Surg (Lond). 2019 Apr 24;42:7-10. doi: 10.1016/j.amsu.2019.04.006. eCollection 2019 Jun.
Nowadays, surgery is considered as one of the most important treatments for hydatidosis. Due to laceration, the cyst and spread of the content within it (protoscoleces) during the surgery that can put the patient at the risk of re-infection, anaphylaxis shock and even death, surgeons use some chemical drugs as protoscolicidal agents. The study is aimed to evaluate the scolicidal effects of olive ( L.) leaf extract on hydatid cyst protoscoleces and .
After the collection of protoscoleces from sheep livers infected with fertile hydatid cysts, they were treated with various concentrations of olive leave extract (75-300 mg/mL) for 5-30 min and . Finally, the mortality of protoscoleces was assessed by the eosin exclusion test (0.1% eosin staining).
The mean of the mortality of protoscoleces was 100% after 10 min of incubation with the concentration of 300 mg/ml of leaves extract. On the other hand, the mean of the mortality of protoscoleces after 20 min of incubation with the concentration of 150 mg/ml of leaves extract was 100%. After injection of leaves extract directly into the hydatid cyst (), the mean of the mortality of protoscoleces was 100% after 12 and 25 min of incubation with the concentration of 300 and 150 mg/ml of leaves extract, respectively; indicating that the extract requiring a further time to display a potent protoscolicidal effects.
Based on the findings of the study, it can be concluded that the extract of olive leaf had a significant scolicidal activity on hydatid cyst protoscoleces. However, further research, especially in human and animal subjects, are required to reach this conclusion.
如今,手术被认为是治疗包虫病最重要的方法之一。由于手术过程中囊肿的撕裂以及囊内容物(原头节)的扩散会使患者面临再次感染、过敏性休克甚至死亡的风险,外科医生会使用一些化学药物作为原头节杀灭剂。本研究旨在评估油橄榄叶提取物对包虫囊肿原头节的杀原头节作用。
从感染有成熟包虫囊肿的绵羊肝脏中收集原头节,然后用不同浓度(75 - 300毫克/毫升)的油橄榄叶提取物处理5 - 30分钟。最后,通过伊红排斥试验(0.1%伊红染色)评估原头节的死亡率。
用浓度为300毫克/毫升的油橄榄叶提取物孵育10分钟后,原头节的平均死亡率为100%。另一方面,用浓度为150毫克/毫升的油橄榄叶提取物孵育20分钟后,原头节的平均死亡率为100%。将油橄榄叶提取物直接注入包虫囊肿后,分别用浓度为300毫克/毫升和150毫克/毫升的油橄榄叶提取物孵育12分钟和25分钟后,原头节的平均死亡率为100%;这表明提取物需要更长时间才能显示出有效的杀原头节作用。
基于该研究结果,可以得出油橄榄叶提取物对包虫囊肿原头节具有显著的杀原头节活性。然而,需要进一步的研究,尤其是在人类和动物受试者中进行研究,才能得出这一结论。