水果对小鼠模型中包虫囊肿原头蚴活力的影响及其急性毒性
Efficacy of Fruits on Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model.
作者信息
Mahmoudvand Hossein, Mirbadie Seyed Reza, Ghasemi Kia Mehdi, Badparva Ebrahim, Shamsadini Lori Saeedeh, Fasihi Harandi Majid
机构信息
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
出版信息
Iran J Parasitol. 2016 Jul-Sep;11(3):383-388.
BACKGROUND
This investigation aimed to evaluate the scolicidal effects of methanolic extract against protoscoleces of hydatid cysts and its acute toxicity in mice NMRI model.
METHODS
Protoscoleces were aseptically extracted from sheep livers having hydatid cysts. Various concentrations of the essential oil (12.5-100 mg/mL) were used for 10 to 60 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Twenty-four male NMRI mice were used to assess the acute toxicity of
RESULTS
extract at the concentrations of 100 mg/mL after 10 min of exposure killed 100% of protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 20 min of exposure to the concentration of 50 mg/mL was 100%. The LD of the intraperitoneal injection of the methanolic extract was 2.8 g/kg and the maximum non-fatal dose was 1.7 g/kg.
CONCLUSION
The findings demonstrated effective scolicidal effects of extract with no considerable toxicity that might be a natural source for the producing of new scolicidal agent.
背景
本研究旨在评估甲醇提取物对包虫囊肿原头蚴的杀原头蚴作用及其在NMRI小鼠模型中的急性毒性。
方法
从患有包虫囊肿的绵羊肝脏中无菌提取原头蚴。使用不同浓度的精油（12.5 - 100毫克/毫升）作用10至60分钟。使用伊红排斥试验（0.1%伊红染色）确认原头蚴的活力。使用24只雄性NMRI小鼠评估提取物在100毫克/毫升浓度下的急性毒性。
结果
暴露10分钟后，100毫克/毫升浓度的提取物杀死了100%的原头蚴。同样，暴露20分钟后，50毫克/毫升浓度下原头蚴的平均死亡率为100%。腹腔注射甲醇提取物的半数致死量为2.8克/千克，最大非致死剂量为1.7克/千克。
结论
研究结果表明提取物具有有效的杀原头蚴作用，且无明显毒性，可能是生产新型杀原头蚴剂的天然来源。
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