Alizadegan Farhood, Aghaei Mahdi, Kumar Sharma J, Saadatmand Massumeh, Kumar Suja Ajoy
Department of Pharmaceutical Sciences, Rajiv Gandhi University of Health Sciences, Bangalore, India.
Department of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran.
J Parasit Dis. 2023 Sep;47(3):683-688. doi: 10.1007/s12639-023-01614-6. Epub 2023 Jul 10.
Due to the increasing resistance to common medicinal compounds, the use of medicinal plants has received special attention. Therefore, the current survey was designed to study the antileishmanial effects of Trautv. methanolic extract against In this study, after preparing the methanolic extract of , its effect on the amastigotes of and triggering the nitric oxide (NO) were measured. Then, the in vivo effect of the methanol extract on cutaneous leishmaniasis in mice was evaluated. The best anti-amastigote effect was for the methanol extract of along with meglumine antimony with 50% inhibitory concentrations value of 17.4 μg/ml ( < 0.001). The 50% cytotoxic concentrations values of methanol extract, meglumine antimoniate, and methanol extract + meglumine antimoniate were 596.3, 784.6, and 296.4 µg/ml, respectively. Macrophages treated with the methanolic extract markedly ( < 0.001) induced the release of nitric oxide. After 28 days of treatment, lesions were completely ( < 0.001) healed in mice treated with the methanolic extract (100 mg/kg) + meglumine antimoniate (25 mg/kg). methanolic extract mainly in combination with meglumine antimoniate showed favorable antileishmanial effects on , concluding that the methanolic extract of can be used for the production of new leishmanicidal agents agaist cutaneous leishmaniasis. Although we revealed that NO trigerring and inhibition of infection in host cells are the antileishmanial mechanism action of methanolic extract, more studies must be performed to clear the mechanisms and its safety.
由于对常见药用化合物的耐药性不断增加，药用植物的使用受到了特别关注。因此，本次调查旨在研究Trautv.甲醇提取物对的抗利什曼原虫作用。在本研究中，制备了的甲醇提取物后，测量了其对的无鞭毛体的作用以及一氧化氮（NO）的释放。然后，评估了甲醇提取物对小鼠皮肤利什曼病的体内作用。对的甲醇提取物与葡甲胺锑联合使用时抗无鞭毛体效果最佳，50%抑制浓度值为17.4μg/ml（<0.001）。甲醇提取物、葡甲胺锑酸盐以及甲醇提取物+葡甲胺锑酸盐的50%细胞毒性浓度值分别为596.3、784.6和296.4μg/ml。用甲醇提取物处理的巨噬细胞显著（<0.001）诱导了一氧化氮的释放。治疗28天后，用甲醇提取物（100mg/kg）+葡甲胺锑酸盐（25mg/kg）处理的小鼠病变完全（<0.001）愈合。主要与葡甲胺锑酸盐联合使用的甲醇提取物对显示出良好的抗利什曼原虫作用，得出结论，的甲醇提取物可用于生产抗皮肤利什曼病的新型杀利什曼原虫剂。尽管我们揭示了NO触发和抑制宿主细胞感染是甲醇提取物的抗利什曼原虫作用机制，但仍需进行更多研究以明确其机制及其安全性。
