Kelley K A, Chamberlain J W, Nolan J A, Horwich A L, Kalousek F, Eisenstadt J, Herrup K, Rosenberg L E
Department of Human Genetics, Yale University School of Medicine, New Haven, Connecticut.
Mol Cell Biol. 1988 Apr;8(4):1821-5. doi: 10.1128/mcb.8.4.1821-1825.1988.
In an attempt to use mouse metallothionein-I (mMT-I) regulatory sequences to direct expression of human ornithine transcarbamylase in the liver of transgenic animals, fusion genes joining either 1.6 kilobases or 185 base pairs of the mMT-I regulatory region to the human ornithine transcarbamylase protein-coding sequence were used to produce transgenic mice. In mice carrying the fusion gene with 1.6 kilobases of the mMT-I 5'-flanking sequences, transgene expression was observed in a wide range of tissues, but, unexpectedly, expression in liver was never observed. Surprisingly, in mice carrying the fusion gene regulated by only 185 base pairs of the mMT-I 5'-flanking sequences, the transgene was expressed exclusively in male germ cells during the tetraploid, pachytene stage of meiosis.
为了利用小鼠金属硫蛋白-I（mMT-I）调控序列在转基因动物肝脏中指导人鸟氨酸转氨甲酰酶的表达，将mMT-I调控区的1.6千碱基或185个碱基对与人类鸟氨酸转氨甲酰酶蛋白编码序列连接的融合基因被用于产生转基因小鼠。在携带具有1.6千碱基mMT-I 5'侧翼序列的融合基因的小鼠中，在多种组织中观察到转基因表达，但出乎意料的是，从未在肝脏中观察到表达。令人惊讶的是，在仅由185个碱基对的mMT-I 5'侧翼序列调控的融合基因的小鼠中，转基因仅在减数分裂的四倍体粗线期雄性生殖细胞中表达。
