大肠杆菌位点特异性DNA倒位的宿主因子：fis基因的克隆与特性分析
Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene.
作者信息
Koch C, Vandekerckhove J, Kahmann R
机构信息
Max-Planck-Institut für Molekulare Genetik, Otto-Warburg-Laboratorium, Berlin, Federal Republic of Germany.
出版信息
Proc Natl Acad Sci U S A. 1988 Jun;85(12):4237-41. doi: 10.1073/pnas.85.12.4237.
Abstract
The Escherichia coli (Es. coli) protein Fis (factor for inversion stimulation) stimulates site-specific DNA inversion of the G segment in phage Mu by binding to a recombinational enhancer. By using synthetic oligonucleotides deduced from the amino-terminal amino acid sequence, we have cloned the gene (termed fis) encoding this specific DNA-binding protein. The DNA sequence shows that the Fis protein is basic and contains 98 amino acids. A helix-turn-helix sequence motif characteristic of many DNA-binding proteins is located at the carboxyl-terminal end of the protein. By marker exchange, we have constructed an insertion mutation of fis. Fis is nonessential for Es. coli growth; however, inversion of the G segment of a Mu prophage was not detected in the fis mutant. The fis gene is located between 71 and 72 min on the Es. coli genetic map.
摘要
大肠杆菌（Es. coli）的Fis蛋白（倒位刺激因子）通过与重组增强子结合，刺激噬菌体Mu中G片段的位点特异性DNA倒位。利用从氨基末端氨基酸序列推导出来的合成寡核苷酸，我们克隆了编码这种特异性DNA结合蛋白的基因（称为fis）。DNA序列表明，Fis蛋白呈碱性，含有98个氨基酸。许多DNA结合蛋白特有的螺旋-转角-螺旋序列基序位于该蛋白的羧基末端。通过标记交换，我们构建了fis的插入突变体。Fis对大肠杆菌生长并非必需；然而，在fis突变体中未检测到Mu原噬菌体G片段的倒位。fis基因位于大肠杆菌遗传图谱上71至72分钟之间。
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Proc Natl Acad Sci U S A. 1988 Jun;85(12):4237-41. doi: 10.1073/pnas.85.12.4237.
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