Mertens G, Fuss H, Kahmann R
J Biol Chem. 1986 Nov 25;261(33):15668-72.
The host range of bacteriophage Mu is regulated through an invertible segment. Inversion requires the presence of two properly oriented recombination sites and a recombinational enhancer sis. The reaction is catalyzed by the Mu-encoded DNA invertase Gin and a host factor termed factors for inversion stimulation (FISs). We present a novel purification scheme for Gin. Purified Gin alone catalyzes the inversion reaction at very low efficiency recombining less than 0.8% of substrate molecules. When supplemented with FIS substrates containing the recombinational enhancer are recombined efficiently. Stoichiometric amounts of Gin are required for recombination.
噬菌体Mu的宿主范围是通过一个可倒位片段来调控的。倒位需要两个方向正确的重组位点和一个重组增强子sis的存在。该反应由Mu编码的DNA转化酶Gin和一种称为倒位刺激因子（FISs）的宿主因子催化。我们提出了一种新的Gin纯化方案。单独纯化的Gin催化倒位反应的效率非常低，重组的底物分子不到0.8%。当补充有包含重组增强子的FIS底物时，底物能高效重组。重组需要化学计量的Gin。
