Zychowska Małgorzata, Półrola Paweł, Chruściński Grzegorz, Zielińska Jolanta, Góral-Półrola Jolanta
Gdansk University of Physical Education and Sport, Department of Life Sciences, Gdansk, Poland.
Jan Kochanowski University in Kielce, Department of Medicine and Health, Kielce, Poland.
Ann Agric Environ Med. 2017 Mar 21;24(1):104-107. doi: 10.5604/12321966.1233977.
Heat stress induces the expression of genes encoding heat-shock proteins and immune response mediators. The aim of this study was to determine the differences in the expression of genes encoding heat-shock proteins 70 kDa and27 kDa, interleukin 6, interleukin 10and C-reactive protein, between athletes and non-athletes after sauna bathing.
Athletes (n=9) and non-athletes (n=9) were exposed to a Finnish sauna twice during one session at a temperature of 98.2 °C and humidity of 10% ± 2%, with a 5 min break for cooling down under a shower. The groups did not differ in terms of age, height or body mass. Blood samples were taken before and after sauna exposure in order to assess gene expression, using reverse transcription polymerase chain reaction.
Differences were observed in leukocyte mRNA levels of tested genes between athletes and non-athletes. In the non-athlete group, all the tested genes were expressed at higher levels as a response to the same heat challenge.
It appears that expression of stress-related genes induced by heat stress is dependent on the level of physical activity.
热应激可诱导编码热休克蛋白和免疫反应介质的基因表达。本研究旨在确定桑拿浴后运动员与非运动员之间编码70 kDa和27 kDa热休克蛋白、白细胞介素6、白细胞介素10和C反应蛋白的基因表达差异。
运动员（n = 9）和非运动员（n = 9）在一次训练中两次暴露于芬兰桑拿房，温度为98.2°C，湿度为10%±2%，中间有5分钟休息时间用于淋浴降温。两组在年龄、身高或体重方面无差异。在桑拿暴露前后采集血样，采用逆转录聚合酶链反应评估基因表达。
观察到运动员与非运动员之间受试基因的白细胞mRNA水平存在差异。在非运动员组中，作为对相同热应激的反应，所有受试基因均以更高水平表达。
热应激诱导的应激相关基因表达似乎取决于体力活动水平。
