The Peter Harrison Centre for Disability Sport, Loughborough University, Loughborough, UK.
School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK.
Cell Stress Chaperones. 2019 Mar;24(2):461-467. doi: 10.1007/s12192-019-00972-6. Epub 2019 Feb 12.
The acute inflammatory response to active or passive activities that increase body temperature may aid to reduce chronic low-grade inflammation. This study investigates the impact of temperature and extracellular heat shock protein 72 (eHsp72) on the acute intracellular Hsp72 (iHsp72) and interleukin-6 (iIL-6) response in monocytes. Whole blood was incubated for 2 h at 37.0 °C, 38.5 °C and 40.0 °C, in the absence or presence of 0.5 μg/ml eHsp72. Flow cytometry was used to assess iHsp72 and iIL-6 expression in total monocytes and the three monocyte subsets. Incubation at 40.0 °C (p < 0.001) but not 38.5 °C (p = 0.085) increased iHsp72 expression when compared with 37.0 °C, while there was no effect of temperature on iIL-6 expression (p = 0.635). Following incubation with eHsp72, the expression of iHsp72 in classical monocytes was reduced at all temperatures (p < 0.001), while there was no effect of eHsp72 on iIL-6 expression (p = 0.071). Large temperature elevations are needed to induce an acute iHsp72 response in monocytes. In addition, contrary to its suggested role as a danger signal for the innate immune system, eHsp72 reduced iHsp72 and iIL-6 expression in monocytes.
主动或被动活动导致体温升高会引起急性炎症反应，这可能有助于减轻慢性低度炎症。本研究旨在探讨温度和细胞外热休克蛋白 72（eHsp72）对单核细胞内热休克蛋白 72（iHsp72）和白细胞介素-6（iIL-6）急性反应的影响。将全血在 37.0°C、38.5°C 和 40.0°C 下孵育 2 小时，在不存在或存在 0.5μg/ml eHsp72 的情况下，使用流式细胞术评估总单核细胞和三种单核细胞亚群中 iHsp72 和 iIL-6 的表达。与 37.0°C 相比，40.0°C（p<0.001）但不是 38.5°C（p=0.085）孵育可增加 iHsp72 的表达，而温度对 iIL-6 表达没有影响（p=0.635）。孵育 eHsp72 后，所有温度下经典单核细胞中 iHsp72 的表达均降低（p<0.001），而 eHsp72 对 iIL-6 表达没有影响（p=0.071）。需要较大的温度升高才能诱导单核细胞发生急性 iHsp72 反应。此外，与作为先天免疫系统危险信号的预期作用相反，eHsp72 降低了单核细胞中 iHsp72 和 iIL-6 的表达。
