Nutrition-Gut-Brain Interactions Research Centre, Faculty of Medicine and Health, School of Medical Sciences, Örebro University, 701 82, Örebro, Sweden.
Man-Technology-Environment (MTM) Research Centre, School of Science and Technology, Örebro University, 701 82, Örebro, Sweden.
Sci Rep. 2021 Jul 30;11(1):15514. doi: 10.1038/s41598-021-94814-0.
The intestinal barrier plays a crucial role in maintaining gut health, and an increased permeability has been linked to several intestinal and extra-intestinal disorders. There is an increasing demand for interventions aimed at strengthening this barrier and for in vivo challenge models to assess their efficiency. This study investigated the effect of sauna-induced dehydration on intestinal barrier function (clinicaltrials.gov: NCT03620825). Twenty healthy subjects underwent three conditions in random order: (1) Sauna dehydration (loss of 3% body weight), (2) non-steroidal anti-inflammatory drug (NSAID) intake, (3) negative control. Intestinal permeability was assessed by a multi-sugar urinary recovery test, while intestinal damage, bacterial translocation and cytokines were assessed by plasma markers. The sauna dehydration protocol resulted in an increase in gastroduodenal and small intestinal permeability. Presumably, this increase occurred without substantial damage to the enterocytes as plasma intestinal fatty acid-binding protein (I-FABP) and liver fatty acid-binding protein (L-FABP) were not affected. In addition, we observed significant increases in levels of lipopolysaccharide-binding protein (LBP), IL-6 and IL-8, while sCD14, IL-10, IFN-ɣ and TNF-α were not affected. These results suggest that sauna dehydration increased intestinal permeability and could be applied as a new physiological in vivo challenge model for intestinal barrier function.
肠道屏障在维持肠道健康方面起着至关重要的作用，其通透性的增加与几种肠道和肠道外疾病有关。人们对旨在增强这种屏障的干预措施以及用于评估其效率的体内挑战模型的需求日益增加。本研究调查了桑拿引起的脱水对肠道屏障功能的影响（clinicaltrials.gov：NCT03620825）。20 名健康受试者以随机顺序接受三种条件：（1）桑拿脱水（体重损失 3%），（2）非甾体抗炎药（NSAID）摄入，（3）阴性对照。通过多糖尿回收试验评估肠道通透性，同时通过血浆标志物评估肠道损伤、细菌易位和细胞因子。桑拿脱水方案导致胃十二指肠和小肠通透性增加。推测这种增加是在肠细胞没有明显损伤的情况下发生的，因为血浆肠脂肪酸结合蛋白（I-FABP）和肝脂肪酸结合蛋白（L-FABP）没有受到影响。此外，我们观察到脂多糖结合蛋白（LBP）、IL-6 和 IL-8 的水平显著升高，而 sCD14、IL-10、IFN-γ和 TNF-α不受影响。这些结果表明，桑拿脱水增加了肠道通透性，可以作为肠道屏障功能的新的生理体内挑战模型。
