Sun Wenyan, Zhang Li, Hou Lin, Ju Chuanxia, Zhao Shengmin, Wei Yaoyue
Department of Biochemistry, Medical College, Qingdao University, Qingdao, China.
Anticancer Drugs. 2017 Jul;28(6):645-653. doi: 10.1097/CAD.0000000000000505.
Isatin was reported to possess anticancer activities through its effect on tumor proliferation, apoptosis, and metastasis in vitro and in vivo. This study aimed to elucidate the underlying mechanism behind isatin's ability to inhibit neuroblastoma cell metastasis. Our results demonstrated that isatin could inhibit neuroblastoma cell proliferation, invasion, and migration in a dose-dependent manner. Moreover, isatin inhibited the expression level of monoamine oxidase A as well as that of its downstream protein hypoxia-inducible factor 1α. Further study indicated that isatin inhibited reactive oxygen species production, extracellular signal-regulated kinase activation, vascular endothelial growth factor receptor-1 phosphorylation, and chemokine receptor type 4 expression. All results support the potential antimetastatic effect of isatin in neuroblatoma cells.
据报道，异吲哚酮通过其在体外和体内对肿瘤增殖、凋亡和转移的影响而具有抗癌活性。本研究旨在阐明异吲哚酮抑制神经母细胞瘤细胞转移能力背后的潜在机制。我们的结果表明，异吲哚酮可以剂量依赖性方式抑制神经母细胞瘤细胞的增殖、侵袭和迁移。此外，异吲哚酮抑制单胺氧化酶A及其下游蛋白缺氧诱导因子1α的表达水平。进一步的研究表明，异吲哚酮抑制活性氧的产生、细胞外信号调节激酶的激活、血管内皮生长因子受体-1的磷酸化以及趋化因子受体4型的表达。所有结果均支持异吲哚酮在神经母细胞瘤细胞中的潜在抗转移作用。
