Tao Yu, Li Mingqiang, Kim Bumjun, Auguste Debra T
Department of Biomedical Engineering, The City College of New York, 160 Convent Avenue, New York, NY 10031, United States.
Department of Biomedical Engineering, Columbia University, New York, NY 10027, United States.
Theranostics. 2017 Feb 8;7(4):899-911. doi: 10.7150/thno.17927. eCollection 2017.
Breast cancer is the second leading cause of cancer-related mortality in women. Successful development of sensitive nanoprobes for breast cancer cell detection is of great importance for breast cancer diagnosis and symptomatic treatment. Herein, inspired by the intrinsic peroxidase property of gold nanoclusters, high loading, and targeting ability of ErbB2/Her2 antibody functionalized liposomes, we report that gold nanoclusters-loaded, target-directed, functionalized liposomes can serve as a robust sensing platform for amplified colorimetric detection of HER2-positive breast cancer cells. This approach allows HER2-positive breast cancer cell identification at high sensitivity with high selectivity. In addition, the colorimetric "readout" offers extra advantages in terms of low-cost, portability, and easy-to-use applications. The practicality of this platform was further proved by successful detection of HER2-positive breast cancer cells in human serum samples and in breast cancer tissue, which indicated our proposed method has potential for application in cancer theranostics.
乳腺癌是女性癌症相关死亡的第二大主要原因。成功开发用于乳腺癌细胞检测的灵敏纳米探针对于乳腺癌的诊断和对症治疗至关重要。在此，受金纳米簇的固有过氧化物酶特性、ErbB2/Her2抗体功能化脂质体的高负载量和靶向能力的启发，我们报道了负载金纳米簇、靶向定向的功能化脂质体可作为用于HER2阳性乳腺癌细胞比色扩增检测的强大传感平台。这种方法能够以高灵敏度和高选择性鉴定HER2阳性乳腺癌细胞。此外，比色“读出”在低成本、便携性和易于使用的应用方面具有额外优势。通过在人血清样本和乳腺癌组织中成功检测HER2阳性乳腺癌细胞，进一步证明了该平台的实用性，这表明我们提出的方法在癌症诊疗方面具有应用潜力。
