Qu Le, Jiang Miao, Qiu Wenjuan, Lu Shiqi, Zhao Yunxiao, Xia Lijun, Ruan Changgeng, Zhao Yiming
Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Collaborative Innovation Center of Hematology, Suzhou, China.
Department of Neurology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Clin Appl Thromb Hemost. 2016 Apr;22(3):252-9. doi: 10.1177/1076029615583347. Epub 2015 Apr 27.
Raised levels of von Willebrand factor (VWF) and reduced levels of a disintegrin and a metalloproteinase with a thrombospondin type I motif, member 13 (ADAMTS13) activity are associated with thrombosis. We aimed to investigate the relationships between plasma levels of VWF and ADAMTS13, their ratios, and the occurrence of cerebral infarction and to understand the roles of VWF and ADAMTS13 in cerebral infarction.
Ninety-four patients with cerebral infarction and 103 controls were analyzed. Plasma levels of VWF antigen (VWF: Ag), VWF ristocetin cofactor activity (VWF: Rcof), and VWF collagen binding activity (VWF: CB) were measured by enzyme-linked immunosorbent assay (ELISA). The ADAMTS13 activity (ADAMTS13) was measured with FRETS-VWF73. The relationship between plasma levels and ratios of VWF and ADAMTS13 and the occurrence of cerebral infarction were analyzed.
Patients with cerebral infarction displayed higher VWF: Ag and VWF: Rcof levels and lower ADAMTS13, VWF: CB/VWF: Ag, ADAMTS13/VWF: Ag, and ADAMTS13/VWF: Rcof levels compared to controls (P < .01). The highest quartiles of VWF: Ag (odds ratio [OR] = 5.11, 95% confidence interval [CI], 1.49-17.50) and VWF: Rcof (OR = 5.04, 95% CI, 1.62-15.66) and the lowest quartiles of VWF: CB/VWF: Ag (OR = 5.91, 95% CI, 1.95-17.93), ADAMTS13/VWF: Ag (OR = 9.11, 95% CI, 2.49-33.33), and ADAMTS13/VWF: Rcof (OR = 3.73, 95% CI, 1.39-10.03) are associated with cerebral infarction.
An association was found between reduced levels of VWF: CB/VWF: Ag, ADAMTS13/VWF: Ag, and ADAMTS13/VWF: Rcof ratios and cerebral infarction. Our data suggest that increased levels of VWF and reduced levels of ADAMTS13 activity may contribute to the pathogenesis of cerebral infarction.
血管性血友病因子（VWF）水平升高以及具有I型血小板反应蛋白基序的解聚素和金属蛋白酶13（ADAMTS13）活性降低与血栓形成有关。我们旨在研究VWF和ADAMTS13的血浆水平、它们的比值与脑梗死发生之间的关系，并了解VWF和ADAMTS13在脑梗死中的作用。
分析了94例脑梗死患者和103例对照。采用酶联免疫吸附测定（ELISA）法测定血浆VWF抗原（VWF:Ag）、VWF瑞斯托霉素辅因子活性（VWF:Rcof）和VWF胶原结合活性（VWF:CB）水平。用FRETS-VWF73测定ADAMTS13活性（ADAMTS13）。分析了VWF和ADAMTS13的血浆水平及比值与脑梗死发生之间的关系。
与对照组相比，脑梗死患者的VWF:Ag和VWF:Rcof水平较高，而ADAMTS13、VWF:CB/VWF:Ag、ADAMTS13/VWF:Ag和ADAMTS13/VWF:Rcof水平较低（P <.01）。VWF:Ag的最高四分位数（比值比[OR]=5.11，95%置信区间[CI]，1.49-17.50）和VWF:Rcof（OR = 5.04，95% CI，1.62-15.66）以及VWF:CB/VWF:Ag的最低四分位数（OR = 5.91，95% CI，1.95-17.93）、ADAMTS13/VWF:Ag（OR = 9.11，95% CI，2.49-33.33）和ADAMTS13/VWF:Rcof（OR = 3.73，95% CI，1.39-10.03）与脑梗死相关。
发现VWF:CB/VWF:Ag、ADAMTS13/VWF:Ag和ADAMTS13/VWF:Rcof比值降低与脑梗死之间存在关联。我们的数据表明，VWF水平升高和ADAMTS13活性降低可能有助于脑梗死的发病机制。
