[核酸杂交技术检测子宫颈癌组织中单纯疱疹病毒Ⅱ型DNA对应序列的初步研究]
[Preliminary study on the nucleic acid hybridization technic in detecting HSV-2 DNA corresponding sequence of cancer tissues of the uterine cervix].
作者信息
Cui H Y, Zhang W, Li J, Liu B Y, Li J S, Lin Y C, Zhang W H, Wu A R, Liu Z M
机构信息
Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing.
出版信息
Zhonghua Zhong Liu Za Zhi. 1987 Sep;9(5):342-4.
In this paper, 34 samples, 25 from 18 cervical carcinoma patients and 9 from 8 non-cervical carcinoma patients, were assayed using (alpha-32P) dCTP HSV-2 DNA probe and nucleic acid hybridization technic. It was shown that the positive rates of HSV-2 DNA corresponding sequence were 50% (2/4), 36% (5/14) and 12% (1/8) in the in situ cancer, invasive cancer and non-cervical cancer tissues. No HSV was isolated from 7 cancer specimens which showed a positive reaction by nucleic acid hybridization.
本文采用（α-32P）dCTP单纯疱疹病毒2型（HSV-2）DNA探针及核酸杂交技术，对34份标本进行检测，其中25份来自18例宫颈癌患者，9份来自8例非宫颈癌患者。结果显示，原位癌、浸润癌及非癌组织中HSV-2 DNA对应序列的阳性率分别为50%（2/4）、36%（5/14）和12%（1/8）。7份核酸杂交呈阳性反应的癌组织标本未分离出HSV。
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