Eglin R P, Sharp F, MacLean A B, Macnab J C, Clements J B, Wilkie N M
Cancer Res. 1981 Sep;41(9 Pt 1):3597-603.
Nonneoplastic and neoplastic cervical biopsy specimens were examined by in situ hybridization to 125I-labeled DNA of herpes simplex virus (HSV), adenovirus, and bacteriophage lambda DNA's, and quantitative hybridization data were obtained using a Video Image Analyser. HSV-specific RNA was detected in 72% of cervical intraepithelial neoplasia, 60% of squamous cervical carcinomas, 2% of nonneoplastic cervices, and 9% of primary adenocarcinomas of the cervix. None of the tissues gave positive hybridization with adenovirus or lambda DNA probes. In paired biopsies of cervical intraepithelial neoplasia and nonneoplastic epithelium from 29 individuals, HSV-specific RNA was detected only in the epithelium of the neoplastic sample and not in the nonneoplastic control. Infectious HSV-2 was isolated from a low proportion (2%) of both ectocervical swabs and cell-free tissue extracts of patients examined, suggesting that the HSV-specific RNA detected in squamous cell neoplasms was not due to overt infections.
通过对单纯疱疹病毒（HSV）、腺病毒和噬菌体λ DNA 的 125I 标记 DNA 进行原位杂交，检查非肿瘤性和肿瘤性宫颈活检标本，并使用视频图像分析仪获得定量杂交数据。在 72% 的宫颈上皮内瘤变、60% 的宫颈鳞状细胞癌、2% 的非肿瘤性宫颈组织以及 9% 的宫颈原发性腺癌中检测到 HSV 特异性 RNA。所有组织与腺病毒或λ DNA 探针均未出现阳性杂交。在 29 名个体的宫颈上皮内瘤变和非肿瘤性上皮的配对活检中，仅在肿瘤性样本的上皮中检测到 HSV 特异性 RNA，而非肿瘤性对照中未检测到。从所检查患者的低比例（2%）宫颈外拭子和无细胞组织提取物中分离出传染性 HSV-2，这表明在鳞状细胞肿瘤中检测到的 HSV 特异性 RNA 并非由于明显感染所致。
