Kajbafzadeh Abdol-Mohammad, Abbasioun Reza, Sabetkish Nastaran, Sabetkish Shabnam, Habibi Ali Akbar, Tavakkolitabassi Kamyar
Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, Tehran, 1419433151, Iran.
Department of Urology and Renal Transplantation, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Int Urol Nephrol. 2017 Jul;49(7):1193-1199. doi: 10.1007/s11255-017-1582-2. Epub 2017 Apr 5.
Few researches have been conducted to develop an ideal method for tissue engineering of corpus cavernosum. We produce a decellularized human corpus cavernosum scaffold and investigate the in vivo cell seeding of the scaffold after transplantation into the rat omentum.
Eight adult human male corpus cavernosum were obtained in sterile condition. After dissecting the urethra, corpus cavernosum was decellularized by inserting an 18-gauge needle into the body of the tissue. The gauge was connected to a peristaltic pump to circulate the detergents in the corpus. Several assessments were performed to evaluate the efficacy of decellularization and extracellular matrix (ECM) preservation. A section of decellularized scaffold was washed several times and transplanted into the omentum of 4 male healthy Sprague-Dawley rats and located into the scrotum. Biopsies were taken 1, 3, and 6 months after transplantation. Histological examination, SEM, and immunohistochemical staining were performed to assess the efficacy of natural recellularization.
The results of the examinations performed prior to transplantation revealed a decellularized ECM resembling to the native tissue with normal pits that may be appropriate for further in vivo cell seeding. Histopathology examination of the biopsies after transplantations confirmed successful cell seeding with endothelium-like cells in different time points. CD34 staining was dominant in the short-time biopsies, while CD31 staining was higher than CD34 in long-term specimens.
The feasibility of natural bioreactor in recellularizing corpus cavernosum was confirmed. This technique may have the potential to facilitate homologous transplantation for repair of corpus defects.
很少有研究致力于开发一种理想的阴茎海绵体组织工程方法。我们制备了一种脱细胞人阴茎海绵体支架，并研究了该支架移植到大鼠大网膜后在体内的细胞接种情况。
在无菌条件下获取8个成年男性阴茎海绵体。分离尿道后，通过将18号针头插入组织体内对阴茎海绵体进行脱细胞处理。该针头连接到蠕动泵以循环组织中的去污剂。进行了多项评估以评价脱细胞效果和细胞外基质（ECM）保存情况。将一段脱细胞支架反复冲洗后移植到4只健康雄性Sprague-Dawley大鼠的大网膜中，并置于阴囊内。在移植后1、3和6个月进行活检。进行组织学检查、扫描电子显微镜检查和免疫组织化学染色以评估自然再细胞化的效果。
移植前的检查结果显示，脱细胞的ECM类似于天然组织，具有正常的凹坑，可能适合进一步进行体内细胞接种。移植后活检的组织病理学检查证实，在不同时间点均成功接种了内皮样细胞。在短期活检中，CD34染色占主导，而在长期标本中，CD31染色高于CD34。
证实了天然生物反应器在阴茎海绵体再细胞化中的可行性。该技术可能具有促进同种异体移植修复阴茎缺陷的潜力。
