Cao Zilong, Liu Liqiang, Jiao Hu, Gan Cheng, Tian Jia, Zhang Tiran, Han Bing
Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Sex Med. 2022 Oct;10(5):100563. doi: 10.1016/j.esxm.2022.100563. Epub 2022 Sep 7.
The autologous skin flap is still the mainstream method for penile reconstruction, but it is very difficult to reconstruct a functional corpus cavernosum. Tissue engineering provides a new idea aiming to restore the damaged or absent corpus cavernosum.
To assess the feasibility of constructing the tissue-engineered corpus cavernosum with autologous adipose stem cells in a rabbit model.
A total of 30 New Zealand male white rabbits. Among them, 20 rabbits were used to obtain the original corpus cavernosum which were used to prepare the acellular corporal scaffolds (ACSs). The others were used for acquiring autologous adipose stem cells (ADSCs) and constructing tissue-engineered corpus cavernosum in vivo.
ACSs were obtained from rabbit penile tissues through an established decellularization procedure. Rabbit autologous ADSCs as seed cells were harvested and expanded. The ADSCs seeded and unseeded ACSs were implanted back into the intramuscular and subcutaneous site in vivo, and the tissue-engineered corpus cavernosum was harvested and analyzed with gross morphology, histological staining, and real-time PCR assay after 1, 3, and 6 months.
ACSs were successfully prepared. The cell non-cytotoxicity and integrity of micro-architecture of ACSs was confirmed in vitro. The cell-seeded scaffold in the intramuscular group was considered as the better strategy for constructing the tissue-engineered corpus cavernosum compared with the other groups. Some α-SMA and CD31 positive cells were detected and identified by immunofluorescent staining and real-time PCR assay in the tissue-engineered corpus cavernosum.
This study provides a new method for constructing the tissue-engineered corpus cavernosum.
First, it is urgent to improve the transformation rate of the endothelial cells and smooth muscle cells from ADSCs. Second, the scaffold harvested in this study was not a complete matrix. Third, further study is needed to explore the potential mechanism of which scaffolds are more suitable for living in intramuscular rather than subcutaneous environment.
In this study, we used the autologous ADSCs as seed cells, the acellular corpus cavernosum as scaffolds, and implanted the grafts back into the rabbit model to preliminarily construct the tissue-engineered corpus cavernosum. This study would provide help for further development in tissue-engineered corpus cavernosum. Cao Z, Liu L, Jiao H, et al. A Preliminary Study of Constructing the Tissue-Engineered Corpus Cavernosum With Autologous Adipose Stem Cells In Vivo. Sex Med 2022;10:100563.
自体皮瓣仍是阴茎重建的主流方法，但重建功能性海绵体非常困难。组织工程学提供了一个旨在修复受损或缺失海绵体的新思路。
评估在兔模型中用自体脂肪干细胞构建组织工程化海绵体的可行性。
共30只新西兰雄性白兔。其中20只用于获取原始海绵体以制备脱细胞海绵体支架（ACSs）。其余用于获取自体脂肪干细胞（ADSCs）并在体内构建组织工程化海绵体。
通过既定的脱细胞程序从兔阴茎组织中获得了ACSs。收获并扩增兔自体ADSCs作为种子细胞。将接种和未接种ADSCs的ACSs植入体内肌肉和皮下部位，1、3和6个月后收获组织工程化海绵体并进行大体形态学、组织学染色和实时PCR分析。
成功制备了ACSs。体外证实了ACSs的细胞无细胞毒性和微观结构完整性。与其他组相比，肌肉内接种细胞的支架被认为是构建组织工程化海绵体的更好策略。通过免疫荧光染色和实时PCR分析在组织工程化海绵体中检测并鉴定出一些α-SMA和CD31阳性细胞。
本研究为构建组织工程化海绵体提供了一种新方法。
第一，迫切需要提高ADSCs向内皮细胞和平滑肌细胞的转化率。第二，本研究中收获的支架不是完整的基质。第三，需要进一步研究以探索哪种支架更适合在肌肉内而非皮下环境中存活的潜在机制。
在本研究中，我们使用自体ADSCs作为种子细胞，脱细胞海绵体作为支架，并将移植物植入兔模型中以初步构建组织工程化海绵体。本研究将为组织工程化海绵体的进一步发展提供帮助。曹Z、刘L、焦H等。体内用自体脂肪干细胞构建组织工程化海绵体的初步研究。性医学2022；10：100563。
