查诺吡喃A：海洋软体动物源真菌的共培养激活了一类罕见的2-烯基-四氢吡喃。

Chaunopyran A: Co-Cultivation of Marine Mollusk-Derived Fungi Activates a Rare Class of 2-Alkenyl-Tetrahydropyran.

作者信息

Shang Zhuo, Salim Angela A, Capon Robert J

机构信息

Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland , St Lucia, QLD 4072, Australia.

出版信息

J Nat Prod. 2017 Apr 28;80(4):1167-1172. doi: 10.1021/acs.jnatprod.7b00144. Epub 2017 Apr 6.

DOI:10.1021/acs.jnatprod.7b00144

PMID:28383912

Abstract

Co-cultivation of Chaunopycnis sp. (CMB-MF028) and Trichoderma hamatum (CMB-MF030), fungal strains co-isolated from the inner tissue of an intertidal rock platform mollusc (Siphonaria sp), resulted in transcriptional activation of a rare class of 2-alkenyl-tetrahydropyran, chaunopyran A (7), and biotransformation and deactivation of the antifungal pyridoxatin (1), to methyl-pyridoxatin (8). This study illustrates the complexity of offensive and counter-offensive molecular defenses encountered during fungal co-cultivation, and the opportunities for activating new, otherwise transcriptionally silent secondary metabolites.

摘要

从潮间带岩石平台软体动物（Siphonaria sp）内部组织中共分离出的真菌菌株Chaunopycnis sp.（CMB-MF028）和哈茨木霉（Trichoderma hamatum，CMB-MF030）共同培养，导致一种罕见的2-烯基四氢吡喃类化合物——chaunopyran A（7）转录激活，同时抗真菌物质吡哆菌素（1）发生生物转化并失活成为甲基吡哆菌素（8）。这项研究说明了真菌共同培养过程中进攻性和反击性分子防御的复杂性，以及激活新的、原本转录沉默的次生代谢产物的机会。