Xu Shuang, Li Mengshi, Hu Zhe, Shao Yilan, Ying Jialiang, Zhang Huawei
School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
Microorganisms. 2023 Feb 12;11(2):464. doi: 10.3390/microorganisms11020464.
Fungi are an important and prolific source of secondary metabolites (SMs) with diverse chemical structures and a wide array of biological properties. In the past two decades, however, the number of new fungal SMs by traditional monoculture method had been greatly decreasing. Fortunately, a growing number of studies have shown that co-culture strategy is an effective approach to awakening silent SM biosynthetic gene clusters (BGCs) in fungal strains to produce cryptic SMs. To enrich our knowledge of this approach and better exploit fungal biosynthetic potential for new drug discovery, this review comprehensively summarizes all fungal co-culture methods and their derived new SMs as well as bioactivities on the basis of an extensive literature search and data analysis. Future perspective on fungal co-culture study, as well as its interaction mechanism, is supplied.
真菌是次生代谢产物（SMs）的重要且丰富的来源，这些次生代谢产物具有多样的化学结构和广泛的生物学特性。然而，在过去二十年中，通过传统单一培养方法获得的新真菌次生代谢产物的数量已大幅减少。幸运的是，越来越多的研究表明，共培养策略是一种有效的方法，可以激活真菌菌株中沉默的次生代谢产物生物合成基因簇（BGCs），从而产生隐秘的次生代谢产物。为了丰富我们对这种方法的认识，并更好地开发真菌的生物合成潜力以用于新药发现，本综述在广泛的文献检索和数据分析的基础上，全面总结了所有真菌共培养方法及其衍生的新次生代谢产物以及生物活性。此外，还提供了真菌共培养研究的未来展望及其相互作用机制。
