Goyon C, Faugeron G, Rossignol J L
Laboratoire I.M.G., Université Paris-Sud, Orsay, France.
Gene. 1988 Mar 31;63(2):297-308. doi: 10.1016/0378-1119(88)90533-1.
We have cloned the met2 gene from Ascobolus immersus by heterologous hybridization with the MET2 gene of Saccharomyces cerevisiae. This gene codes for the homoserine O-transacetylase, one of the methionine biosynthetic enzymes. The complete nucleotide sequence of a 2910-bp DNA fragment carrying the met2 gene has been determined. The gene contains a 165-bp intron which is similar in structure to other fungal introns. The deduced amino acid (aa) sequence (518 aa residues; Mr of 57726) shows three domains with a significant level of homology with the corresponding yeast protein. Northern-blot analysis reveals at least two transcripts (2.4 and 2.1 kb) probably due to transcription termination heterogeneity, as suggested by S1-mapping experiments. Polymorphism has been observed in the met2 gene flanking regions of Ascobolus strains from two different stocks.
我们通过与酿酒酵母的MET2基因进行异源杂交，从浸没巨孢囊霉中克隆出了met2基因。该基因编码高丝氨酸O-转乙酰酶，这是甲硫氨酸生物合成酶之一。已确定了携带met2基因的一个2910 bp DNA片段的完整核苷酸序列。该基因包含一个165 bp的内含子，其结构与其他真菌内含子相似。推导的氨基酸序列（518个氨基酸残基；分子量为57726）显示出三个结构域，与相应的酵母蛋白具有显著的同源性。Northern印迹分析显示至少有两种转录本（2.4和2.1 kb），这可能是由于转录终止异质性所致，正如S1作图实验所表明的那样。在来自两个不同菌株库的浸没巨孢囊霉菌株的met2基因侧翼区域观察到了多态性。
