Langin T, Faugeron G, Goyon C, Nicolas A, Rossignol J L
Gene. 1986;49(3):283-93. doi: 10.1016/0378-1119(86)90364-1.
A 5.1-kb DNA fragment from Saccharomyces cerevisiae, which complements a yeast met2 mutant strain, has been cloned. This fragment contains the wild-type MET2 gene which codes for the homoserine O-transacetylase, one of the methionine biosynthetic enzymes. The presence of the MET2 gene has been shown by integrative transformation experiments and genetic analyses of the resulting transformants. The complete nucleotide sequence of a 2826-bp DNA fragment carrying the MET2 gene has been determined. The sequence contains one major open reading frame of 438 codons, giving a calculated Mr of 48,370 for the encoded protein. We have identified the transcriptional product of the MET2 gene and estimated its size at 1650 nucleotides.
已克隆出一段来自酿酒酵母的5.1千碱基对DNA片段，该片段可互补酵母met2突变菌株。此片段包含野生型MET2基因，该基因编码高丝氨酸O-转乙酰酶，它是甲硫氨酸生物合成酶之一。通过整合转化实验及对所得转化体的遗传分析，已证实MET2基因的存在。已测定了携带MET2基因的2826碱基对DNA片段的完整核苷酸序列。该序列包含一个由438个密码子组成的主要开放阅读框，所编码蛋白质的计算分子量为48370。我们已鉴定出MET2基因的转录产物，并估计其大小为1650个核苷酸。
